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© by The Society for Leukocyte Biology
Journal of Leukocyte Biology, doi:10.1189/jlb.1107797

Received for publication November 27, 2007.
Revised March 3, 2008.
Accepted for publication March 4, 2008.

Article

Phlebotomine salivas inhibit immune inflammation-induced neutrophil migration via an autocrine DC-derived PGE2/IL-10 sequential pathway

Vanessa Carregaro *, Jesus G. Valenzuela {dagger}, Thiago M. Cunha {ddagger}, Waldiceu A. Verri Jr.{ddagger}, Renata Grespan {ddagger}, Graziela Matsumura {ddagger}, José M. C. Ribeiro {sect}, Dia-Eldin Elnaiem {dagger}, João S. Silva *, and Fernando Q. Cunha {ddagger}@

*Department of Biochemistry and Immunology and {ddagger}Pharmacology, School of Medicine of Ribeirão Preto, University of Sao Paulo, Brazil; and {dagger}Vector Molecular Biology Unit and {sect}Vector Biology Section, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA

@ To whom correspondence should be addressed. E-mail: fdqcunha{at}fmrp.usp.br.


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Abstract

In the present study, we investigated whether saliva from Phlebotomus papatasi and Phlebotomus duboscqi inhibited antigen-induced neutrophil migration and the mechanisms involved in these effects. The pretreatment of immunized mice with salivary gland extracts (SGE) of both phlebotomines inhibited OVA challenge-induced neutrophil migration and release of the neutrophil chemotactic mediators, MIP-1{alpha}, TNF-{alpha}, and leukotriene B4 (LTB4). Furthermore, SGE treatment enhanced the production of anti-inflammatory mediators, IL-10 and PGE2. SGE treatments failed to inhibit neutrophil migration and MIP-1{alpha} and LTB4 production in IL-10-/- mice, also failing in mice treated with nonselective (indomethacin) or selective (rofecoxibe) cyclooxygenase (COX) inhibitors. COX inhibition resulted in diminished SGE-induced IL-10 production, and PGE2 release triggered by SGE remained increased in IL-10-/- mice, suggesting that prostanoids are acting through an IL-10-dependent mechanism. SGE treatments in vivo reduced the OVA-induced lymphoproliferation of spleen-derived cells. Further, the in vitro incubation of bone marrow-derived dendritic cells (DC) with SGE inhibited the proliferation of CD4+T cells from OVA-immunized mice, which was reversed by indomethacin and anti-IL-10 antibody treatments. Supporting these results, SGE induced the production of PGE2 and IL-10 by DC, which were blocked by COX inhibition. These effects were associated with the reduction of DC-membrane expression of MHC-II and CD86 by SGE treatment. Altogether, the results showed that Phlebotomine saliva inhibits immune inflammation-induced neutrophil migration by an autocrine DC sequential production of PGE2/IL-10, suggesting that the saliva constituents might be promising therapeutic molecules to target immune inflammatory diseases.

Key Words: antigen presentation • anti-inflammatory mediators • inflammatory diseases • cytokines • insect saliva • sand fly