Journal of Leukocyte Biology eBioscience full spectrum cell analysis
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on June 1, 2007

Published online before print March 16, 2007
This Article
Right arrow Full Text (Reprint (PDF))
Right arrow All Versions of this Article:
jlb.1106672v1
81/6/1568    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ding, Q.
Right arrow Articles by Chen, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ding, Q.
Right arrow Articles by Chen, Y.
© by The Society for Leukocyte Biology
Journal of Leukocyte Biology, doi:10.1189/jlb.1106672

Received for publication November 12, 2006.
Revised January 30, 2007.
Accepted for publication February 12, 2007.

Article

Catalase potentiates retinoic acid-induced THP-1 monocyte differentiation into macrophage through inhibition of peroxisome proliferator-activated receptor {gamma}

Qiurong Ding , Ting Jin , Zhenzhen Wang , and Yan Chen @

Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai, China

@ To whom correspondence should be addressed. E-mail: ychen3{at}sibs.ac.cn.


  Abstract

Macrophage differentiation plays a pivotal role in cardiovascular diseases and many other physiological processes. However, the role of reaction oxygen species in macrophage differentiation has not been elucidated. Here, we report functional characterization of catalase, an enzyme that degrades hydrogen peroxide (H2O2), in THP-1 monocyte differentiation. Treatment of THP-1 cells with catalase was able to synergize with all-trans retinoic acid (ATRA) to enhance macrophage differentiation, demonstrated by changes of cell adherence, cell cycle arrest, nitroblue tetrazolium reduction, and expression of differentiation markers including CD68, CD11b, and matrix metalloproteinase 9 (MMP9). ATRA could stimulate retinoic acid (RA) receptor-mediated transcription, but this was not affected by catalase. However, ATRA and catalase were capable of reducing transcriptional activity mediated by peroxisome proliferator-activated receptor {gamma} (PPAR{gamma}). Consistently, PPAR{gamma} antagonists enhanced, and PPAR{gamma} agonists inhibited MMP9 expression stimulated by ATRA and catalase in THP-1 cells. Therefore, these data indicate that catalase is able to potentiate ATRA-induced macrophage differentiation by inhibition of PPAR{gamma} activity, underscoring an important interplay between H2O2, RA, and PPAR{gamma} in macrophages.

Key Words: PPAR • ROS




This article has been cited by other articles:


Home page
 CirculationHome page
T. Watanabe, K. Nishio, T. Kanome, T.-a. Matsuyama, S. Koba, T. Sakai, K. Sato, S. Hongo, K. Nose, H. Ota, et al.
Impact of Salusin-{alpha} and -{beta} on Human Macrophage Foam Cell Formation and Coronary Atherosclerosis
Circulation, February 5, 2008; 117(5): 638 - 648.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Copyright © 2007 by the Society for Leukocyte Biology.