HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

Published online before print October 13, 2006

This Article Full Text (Reprint (PDF)) All Versions of this Article: jlb.1105696v1 81/1/168    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Kühl, A. A. Articles by Hoffmann, J. C. Search for Related Content PubMed PubMed Citation Articles by Kühl, A. A. Articles by Hoffmann, J. C.

© by The Society for Leukocyte Biology
Journal of Leukocyte Biology, doi:10.1189/jlb.1105696

Received for publication November 29, 2005.
Revised July 26, 2006.
Accepted for publication August 19, 2007.

Article

Aggravation of intestinal inflammation by depletion/deficiency of T cells in different types of IBD animal models

*Medizinische Klinik I, Universitätsmedizin Berlin, Campus Benjamin Franklin, Berlin, Germany; and Institute of Pathology, Charité, University Medicine Berlin, Campus Benjamin Franklin, Berlin, Germany

^@ To whom correspondence should be addressed. E-mail: joerg.hoffmann{at}charite.de.

	Abstract

The role of T cells in inflammatory bowel disease (IBD) is still controversial. Although T cells induce IBD in immunodeficient animals, others suggest a protective role of T cells. Therefore, this study was conducted in order to elucidate the effect of T cell depletion/deficiency on different IBD animal models. Mice depleted of or deficient in T cells were exposed to dextran sodium sulfate (DSS) in order to induce colitis. In addition, T cells were depleted in mice with terminal ileitis (TNF^ARE) or colitis due to interleukin 2 deficiency (IL-2 ko). Finally, DSS-induced colitis was studied in mice deficient in interferon gamma (IFN- ko) upon T cell depletion. Depletion of T cells aggravated DSS-induced colitis and terminal ileitis of TNF^ARE mice. Exacerbated DSS-induced colitis was also found in T cell-deficient mice. IL-2 ko mice showed increased mortality upon early (starting at 4 wk of age) but not late depletion (starting at 8 wk of age). Early T cell depletion or deficiency resulted in increased IFN- production by both lamina propria lymphocytes and splenocytes in every model investigated herein. In IFN- ko mice, T cell depletion did not affect the development and course of DSS-induced colitis. The protective effect of T cells in IBD was confirmed in various IBD animal models. Particularly, during the early phase of intestinal inflammation, T cells appear to be important. The mechanism seems to involve the control of IFN- production and epithelial regeneration.

Key Words: colitis • epithelial regeneration • interferon • terminal ileitis