HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

Published online before print April 14, 2006

This Article Full Text (Reprint (PDF)) All Versions of this Article: jlb.1105683v1 80/1/107    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Fraser, D. A. Articles by Tenner, A. J. Search for Related Content PubMed PubMed Citation Articles by Fraser, D. A. Articles by Tenner, A. J.

© by The Society for Leukocyte Biology
Journal of Leukocyte Biology, doi:10.1189/jlb.1105683

Received for publication November 23, 2005.
Revised February 26, 2006.
Accepted for publication March 10, 2006.

Article

C1q and MBL, components of the innate immune system, influence monocyte cytokine expression

Deborah A. Fraser ^*, Suzanne S. Bohlson ^*, Nijole Jasinskiene ^*, Nenoo Rawal , Gail Palmarini ^*, Sol Ruiz ^*, Rosemary Rochford , and Andrea J. Tenner ^*@

Departments of *Molecular Biology and Biochemistry and Pathology, Center for Immunology, University of California, Irvine; Department of Biochemistry, University of Texas Health Center at Tyler; and Department of Microbiology and Immunology, State University of New York, Upstate Medical University, Syracuse

^@ To whom correspondence should be addressed. E-mail: ATenner{at}uci.edu.

	Abstract

It has recently been recognized that the innate immune response, the powerful first response to infection, has significant influence in determining the nature of the subsequent adaptive immune response. C1q, mannose-binding lectin (MBL), and other members of the defense collagen family of proteins are pattern recognition molecules, able to enhance the phagocytosis of pathogens, cellular debris, and apoptotic cells in vitro and in vivo. Humans deficient in C1q inevitably develop a lupus-like autoimmune disorder, and studies in C1q knockout mice demonstrate a deficiency in the clearance of apoptotic cells with a propensity for autoimmune responses. The data presented here show that under conditions in which phagocytosis is enhanced, C1q and MBL modulate cytokine production at the mRNA and protein levels. Specifically, these recognition molecules of the innate immune system contribute signals to human peripheral blood mononuclear cells, leading to the suppression of lipopolysaccharide-induced proinflammatory cytokines, interleukin (IL)-1 and IL-1, and an increase in the secretion of cytokines IL-10, IL-1 receptor antagonist, monoycte chemoattractant protein-1, and IL-6. These data support the hypothesis that defense collagen-mediated suppression of a proinflammatory response may be an important step in the avoidance of autoimmunity during the clearance of apoptotic cells.

Key Words: human • macrophages • complement

This article has been cited by other articles:

				A. P. Lillis, M. C. Greenlee, I. Mikhailenko, S. V. Pizzo, A. J. Tenner, D. K. Strickland, and S. S. Bohlson Murine Low-Density Lipoprotein Receptor-Related Protein 1 (LRP) Is Required for Phagocytosis of Targets Bearing LRP Ligands but Is Not Required for C1q-Triggered Enhancement of Phagocytosis J. Immunol., July 1, 2008; 181(1): 364 - 373. [Abstract] [Full Text] [PDF]

				R. Ramasawmy, G. S. Spina, K. C. Fae, A. C. Pereira, R. Nisihara, I. J. Messias Reason, M. Grinberg, F. Tarasoutchi, J. Kalil, and L. Guilherme Association of Mannose-Binding Lectin Gene Polymorphism but Not of Mannose-Binding Serine Protease 2 with Chronic Severe Aortic Regurgitation of Rheumatic Etiology Clin. Vaccine Immunol., June 1, 2008; 15(6): 932 - 936. [Abstract] [Full Text] [PDF]

				N. Rawal, R. Rajagopalan, and V. P. Salvi Activation of Complement Component C5: COMPARISON OF C5 CONVERTASES OF THE LECTIN PATHWAY AND THE CLASSICAL PATHWAY OF COMPLEMENT J. Biol. Chem., March 21, 2008; 283(12): 7853 - 7863. [Abstract] [Full Text] [PDF]

				T. Carter, M. Sumiya, K. Reilly, R. Ahmed, P. Sobieszczuk, J. A. Summerfield, and R. A. Lawrence Mannose-Binding Lectin A-Deficient Mice Have Abrogated Antigen-Specific IgM Responses and Increased Susceptibility to a Nematode Infection J. Immunol., April 15, 2007; 178(8): 5116 - 5123. [Abstract] [Full Text] [PDF]

				D. A. Fraser, M. Arora, S. S. Bohlson, E. Lozano, and A. J. Tenner Generation of Inhibitory NF{kappa}B Complexes and Phosphorylated cAMP Response Element-binding Protein Correlates with the Anti-inflammatory Activity of Complement Protein C1q in Human Monocytes J. Biol. Chem., March 9, 2007; 282(10): 7360 - 7367. [Abstract] [Full Text] [PDF]