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Published online before print November 29, 2004

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© by The Society for Leukocyte Biology
Journal of Leukocyte Biology, doi:10.1189/jlb.1004559

Received for publication October 3, 2004.

Accepted for publication October 28, 2004.

Article

The CMRF58 antibody recognizes a subset of CD123^hi dendritic cells in allergen-challenged mucosa

Slavica Vuckovic ^*@, Dalia Khalil ^*, Nicola Angel ^*, Frode Jahnsen , Iona Hamilton ^*, Amanda Boyce ^*, Barry Hock , and Derek N. J. Hart ^*

*Mater Medical Research Institute, South Brisbane, Queensland, Australia; Institute of Pathology, Rikshospitalet, Oslo, Norway; and Hematology/Immunology Research Group, Christchurch Hospital, New Zealand

	Abstract

CD123^hi CD11c^- dendritic cells (CD123^hi DC) are a distinct subset of human DC present in bone marrow, blood, lymphoid organs, and peripheral tissues. Pathogen stimulation, cytokine, or CD40 ligation induces CD123^hi DC maturation, involving a shift from their innate immune to cognate antigen-presenting functions. In this study, we revealed that blood CD123^hi DC in the presence of cytokine (granulocyte macrophage-colony stimulating factor and interleukin-3) undergo progressive, step-wise maturation through an "early" stage, delineated by expression of the antigen detected by the new monoclonal antibody CMRF58 (CD123^hiCMRF58⁺CD40^-CD86^-CD83^-) to the "late" stage with costimulatory antigen expression (CD123^hiCMRF58⁺CD40⁺CD86⁺CD83^+/-). In this early stage, cytokine-maintained CD123^hi DC do not display changes in their morphology, no longer produce interferon- (IFN-) in response to bacteria, and develop the capacity to induce proliferation and polarization of allogeneic T cells. CD123^hiCMRF58⁺ DC, phenotypically similar to in vitro cytokine-maintained CD123^hi DC, were not detected in tonsil but are present in allergen-challenged nasal mucosa of allergic individuals. Thus, CD123^hi DC in certain tissue environments such as allergen-challenged nasal mucosa share a common CD123^hiCMRF58⁺ phenotype with in vitro cytokine-maintained blood CD123^hi DC characterized by lack of IFN- production.

Key Words: mAb • nasal allergy

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