Journal of Leukocyte Biology Myeloid cells, immune suppression, tumor immunology
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Published online before print April 10, 2008
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© by The Society for Leukocyte Biology
Journal of Leukocyte Biology, doi:10.1189/jlb.0907659

Received for publication September 28, 2007.
Revised March 13, 2008.
Accepted for publication March 13, 2008.

Article

Separation of splenic red and white pulp occurs before birth in a LT{alpha}{beta}-independent manner

Mark F. R. Vondenhoff *, Guillaume E. Desanti {dagger}, Tom Cupedo {ddagger}, Julien Y. Bertrand {sect}, Ana Cumano {dagger}, Georg Kraal *, Reina E. Mebius *, and Rachel Golub {dagger}@

{dagger}Unite du Développement des Lymphocytes, INSERM U668, Institut Pasteur, Paris, France; *Department of Molecular Cell Biology and Immunology, VU University Medical Center, Amsterdam, The Netherlands; {ddagger}Erasmus Medical Center, Department of Hematology, Rotterdam, The Netherlands; and {sect}University of California, San Diego, La Jolla, California, USA

@ To whom correspondence should be addressed. E-mail: rgolub{at}pasteur.fr.


  Abstract

For the formation of lymph nodes and Peyer’s patches, lymphoid tissue inducer (LTi) cells are crucial in triggering stromal cells to recruit and retain hematopoietic cells. Although LTi cells have been observed in fetal spleen, not much is known about fetal spleen development and the role of LTi cells in this process. Here, we show that LTi cells collect in a periarteriolar manner in fetal spleen at the periphery of the white pulp anlagen. Expression of the homeostatic chemokines can be detected in stromal and endothelial cells, suggesting that LTi cells are attracted by these chemokines. As lymphotoxin (LT){alpha}1{beta}2 can be detected on B cells but not LTi cells in neonatal spleen, starting at 4 days after birth, the earliest formation of the white pulp in fetal spleen occurs in a LT{alpha}1{beta}2-independent manner. The postnatal development of the splenic white pulp, involving the influx of T cells, depends on LT{alpha}1{beta}2 expressed by B cells.

Key Words: lymphoid tissue inducer cells • organogenesis • embryo • chemokines • stroma • mouse






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