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Published online before print January 24, 2006
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© by The Society for Leukocyte Biology
Journal of Leukocyte Biology, doi:10.1189/jlb.0905512

Received for publication September 12, 2005.
Revised November 16, 2005.
Accepted for publication November 25, 2005.

Article

The lectin jacalin plus costimulation with anti-CD28 antibody induces phosphorylation of p38 MAPK and IL-4 synthesis--I

Seetha M. Lakshmi Tamma *@, Satya Priya Balan *, Ken Wook Chung *, and Savita Pahwa {dagger}

*Department of Biomedical Sciences, C. W. Post Campus, Long Island University, Brookville, New York; and {dagger}University of Miami, School of Medicine, Microbiology and Immunology, Florida

@ To whom correspondence should be addressed. E-mail: stamma{at}liu.edu.


  Abstract

Costimulatory signals play an important role in the development of T helper cell type 1 (Th1) or Th2 type. Little is known about jacalin plus CD28-mediated signaling and cytokine secretion. In the present study, we analyzed the intracellular signaling events following stimulation of CD4+ T cells with jacalin plus CD28 cross-linking (CD28XL) with anti-CD28 antibody. Our results indicate enhanced phosphorylation of Tec and linker for activation of T cells when compared with stimulation with jacalin alone or CD28XL alone. Stimulation with jacalin or CD28XL appears to be insufficient to induce interleukin (IL)-4 secretion; however, CD28XL followed by stimulation with jacalin resulted in enhanced phosphorylation of p38 mitogen-activated protein kinase (MAPK) and increased secretion of IL-4. However, compared with stimulation with phorbol 12-myristate 13-acetate plus ionomycin, jacalin plus CD28XL resulted in decreased levels of tumor necrosis factor {alpha} secretion. Addition of p38 inhibitor, SB203580, inhibited p38 phosphorylation and IL-4 secretion. These data suggest that jacalin stimulation alone appears to be insufficient for Th2 development, and addition of CD28 costimulation induced Th2 generation. We propose that jacalin plus CD28XL induces Th2 differentiation via activation of p38 MAPK.

Key Words: Th1 • CD28XL • PMA




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[Abstract] [Full Text] [PDF]


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