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Published online before print January 2, 2004

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© by The Society for Leukocyte Biology
Journal of Leukocyte Biology, doi:10.1189/jlb.0902471

Received for publication September 25, 2002.
Revised October 30, 2003.
Accepted for publication December 1, 2003.

Article

Expression and synthesis of fibroblast growth factor-9 in human T-lymphocytes. Response to isopentenyl pyrophosphate and TGF-1/IL-15

University Medical Clinic I, Department of Pneumology and Allergy/Immunology, Friedrich-Schiller-University Jena, Germany

^@ To whom correspondence should be addressed. E-mail: grefachew.workalemahu{at}med.uni-jena.de.

	Abstract

T-lymphocytes are believed to play a role in maintaining the normal configuration of epithelial tissue. As little is known about the factors mediating this function, we addressed the question of whether T-lymphocytes produce fibroblast growth factor (FGF)-9 as well as two other growth factors associated with epithelial tissue reconstitution. Blood T cells isolated from healthy donors were grown in the presence of isopentenyl pyrophosphate (IPP) or transforming growth factor-1 (TGF-1)/interleukin-15 (IL-15) for 24 h and were assessed for the expression and synthesis of FGF-9, keratinocyte growth factor (KGF), and epidermal growth factor (EGF). Resting human T cells constitutively expressed KGF and FGF-9 mRNA but no EGF mRNA. In the presence of IPP, FGF-9 mRNA expression significantly increased in a dose-dependent manner, expression of KGF remained unaltered, and EGF mRNA could not be detected. In contrast to IPP, stimulation of the cells with TGF-1/IL-15 did not alter FGF-9 expression. Moreover, stimulation with anti-CD3 does not induce FGF-9 expression but triggers a high signal of interferon- mRNA. Western blot analysis of T cell lysates, prepared 4 days following stimulation with IPP, showed an increase of FGF-9 protein as compared with control cells. In conclusion, the results demonstrate for the first time that human blood and bronchoalveolar lavage T-lymphocytes are capable of expressing FGF-9. The data also provide novel evidence that immunoregulatory cells can synthesize FGF-9.

Key Words: keratinocyte growth factor • epidermal growth factor • BAL

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