Journal of Leukocyte Biology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on June 1, 2003

Published online before print May 22, 2003
This Article
Right arrow Full Text (Reprint (PDF))
Right arrow All Versions of this Article:
jlb.0902443v1
73/6/731    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Stevenaert, F.
Right arrow Articles by Leclercq, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Stevenaert, F.
Right arrow Articles by Leclercq, G.
© 2003 by The Society for Leukocyte Biology
Journal of Leukocyte Biology, doi:10.1189/jlb.0902443

Received for publication September 9, 2002.
Revised February 10, 2003.
Accepted for publication February 12, 2003.

Article

Ly49E expression points toward overlapping but distinct natural killer (NK) cell differentiation kinetics and potential of fetal versus adult lymphoid progenitors

Frederik Stevenaert , Katrien Van Beneden , An De Creus , Veronique Debacker , Jean Plum , and Georges Leclercq @

Department of Clinical Chemistry, Microbiology and Immunology, University of Ghent, University Hospital, Belgium

@ To whom correspondence should be addressed. E-mail: georges.leclercq{at}rug.ac.be.


  Abstract

Using a new antibody, we found previously that contrary to adult natural killer (NK) cells, fetal NK cells have a unique phenotype, as they exclusively express Ly49E. This can be explained by an intrinsic, different NK differentiation potential of fetal versus adult lymphoid progenitors by immaturity of fetal NK cells or by instability of Ly49E expression. Here, we show that adult progenitor cells were still capable of differentiating into Ly49E-expressing NK cells but at a much lower frequency. Surprisingly, Ly49E expression in vitro did not require stromal cells. Kinetic analysis in vivo showed that Ly49E was expressed early, together with CD94/NKG2 and Ly49G2, followed by Ly49C, and finally Ly49D. Transfer of sorted Ly49E-positive, fetal NK cells showed stable Ly49E expression, and later, part of these cells up-regulated other Ly49 members. These data indicate that although there are intrinsic differences, there is no strict fetal and adult wave of NK cell differentiation.

Key Words: cellular differentiation • repertoire development • cell-surface molecules




This article has been cited by other articles:


Home page
 J. Immunol.Home page
F. Gays, K. Martin, R. Kenefeck, J. G. Aust, and C. G. Brooks
Multiple Cytokines Regulate the NK Gene Complex-Encoded Receptor Repertoire of Mature NK Cells and T Cells
J. Immunol., September 1, 2005; 175(5): 2938 - 2947.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
F. Stevenaert, K. Van Beneden, V. De Colvenaer, A. S. Franki, V. Debacker, T. Boterberg, D. Deforce, K. Pfeffer, J. Plum, D. Elewaut, et al.
Ly49 and CD94/NKG2 receptor acquisition by NK cells does not require lymphotoxin-{beta} receptor expression
Blood, August 1, 2005; 106(3): 956 - 962.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
K. Takeda, E. Cretney, Y. Hayakawa, T. Ota, H. Akiba, K. Ogasawara, H. Yagita, K. Kinoshita, K. Okumura, and M. J. Smyth
TRAIL identifies immature natural killer cells in newborn mice and adult mouse liver
Blood, March 1, 2005; 105(5): 2082 - 2089.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Copyright © 2003 by the Society for Leukocyte Biology.