| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
Published online before print November 16, 2006
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Article |
Department of Ophthalmology, Case Western Reserve University, Cleveland, Ohio, USA
@ To whom correspondence should be addressed. E-mail: Eric.Pearlman{at}case.edu.
 |
Abstract |
|---|
The severity of corneal inflammation depends on the activity of infiltrating neutrophils responding to chemotactic factors such as CXC chemokines. This study examines the relative contribution of CXCL1/keratinocyte-derived chemokine (KC), CXCL2/MIP-2, and CXCL5/LPS-induced chemokine (LIX) in neutrophil recruitment to the corneal stroma during LPS keratitis, where neutrophils infiltrate the corneal stroma at 6 h after LPS injection and peak at 24 h. Consistent with this timeframe, KC was detected after 3 h, reached peak levels at 24 h, and decreased thereafter. In contrast, LIX production was not detected until 8 h after injection and peaked at 24 h. MIP-2 was detected at 3 h but did not reach the levels of KC and LIX. Cell types associated with corneal inflammation produced markedly different chemokines in vitro: Murine corneal fibroblasts (MK/T-1) produced LIX and KC in response to LPS but did not produce MIP-2, whereas peritoneal macrophages and neutrophils produced MIP-2 and KC but did not produce LIX. To determine the relative contribution of these chemokines in neutrophil recruitment to the cornea, anti-LIX, anti-KC, or anti-MIP-2 was injected into the corneal stroma of enhanced GFP chimeric mice prior to LPS, and total cell and neutrophil infiltration was examined. Antibody to LIX and KC, injected individually or in combination, significantly inhibited neutrophil recruitment to the cornea, whereas anti-MIP-2 had no inhibitory effect. Together, these findings demonstrate cell-specific production of CXC chemokines and show that LIX and KC mediate neutrophil recruitment into the cornea during LPS keratitis.
Key Words: chemokine • macrophage • inflammation • MIP-2
This article has been cited by other articles:
|
|
 |
|
  R. T. Ramadan, A. L. Moyer, and M. C. Callegan A Role for Tumor Necrosis Factor-{alpha} in Experimental Bacillus cereus Endophthalmitis Pathogenesis Invest. Ophthalmol. Vis. Sci., October 1, 2008; 49(10): 4482 - 4489. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. F. Bento, D. F. P. Leite, R. F. Claudino, D. B. Hara, P. C. Leal, and J. B. Calixto The selective nonpeptide CXCR2 antagonist SB225002 ameliorates acute experimental colitis in mice J. Leukoc. Biol., October 1, 2008; 84(4): 1213 - 1221. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. B. Tarabishy, B. Aldabagh, Y. Sun, Y. Imamura, P. K. Mukherjee, J. H. Lass, M. A. Ghannoum, and E. Pearlman MyD88 Regulation of Fusarium Keratitis Is Dependent on TLR4 and IL-1R1 but Not TLR2 J. Immunol., July 1, 2008; 181(1): 593 - 600. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Lin, P. Jackson, A. M. Tester, E. Diaconu, C. M. Overall, J. E. Blalock, and E. Pearlman Matrix Metalloproteinase-8 Facilitates Neutrophil Migration through the Corneal Stromal Matrix by Collagen Degradation and Production of the Chemotactic Peptide Pro-Gly-Pro Am. J. Pathol., July 1, 2008; 173(1): 144 - 153. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Gillette-Ferguson, K. Daehnel, A. G. Hise, Y. Sun, E. Carlson, E. Diaconu, H. F. McGarry, M. J. Taylor, and E. Pearlman Toll-Like Receptor 2 Regulates CXC Chemokine Production and Neutrophil Recruitment to the Cornea in Onchocerca volvulus/ Wolbachia-Induced Keratitis Infect. Immun., December 1, 2007; 75(12): 5908 - 5915. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Koedel, U. M. Merbt, C. Schmidt, B. Angele, B. Popp, H. Wagner, H.-W. Pfister, and C. J. Kirschning Acute Brain Injury Triggers MyD88-Dependent, TLR2/4-Independent Inflammatory Responses Am. J. Pathol., July 1, 2007; 171(1): 200 - 213. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
