Journal of Leukocyte Biology
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A more recent version of this article appeared on January 1, 2008

Published online before print October 10, 2007
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© by The Society for Leukocyte Biology
Journal of Leukocyte Biology, doi:10.1189/jlb.0607436

Received for publication June 27, 2007.
Revised September 10, 2007.
Accepted for publication September 14, 2007.

Article

Identification and monitoring of effector and regulatory T cells during experimental arthritis based on differential expression of CD25 and CD134

Esther N. M. Nolte-'t Hoen *, Elmieke P. J. Boot {dagger}{ddagger}, Josée P. A. Wagenaar-Hilbers {dagger}, Jolanda H. M. van Bilsen {dagger}, Ger J. A. Arkesteijn {dagger}, Gert Storm {ddagger}, Linda A. Everse {dagger}{ddagger}, Willem van Eden {dagger}, and Marca H. M. Wauben *@

Departments of *Biochemistry and Cell Biology and {dagger}Infectious Diseases and Immunology, Faculty of Veterinary Medicine, and {ddagger}Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands


  Abstract

Major problems in the analysis of CD4+ effector cell and regulatory T cell (Treg) populations in an activated immune system are caused by the facts that both cell types can express CD25 and that the discriminatory marker forkhead box p3 can only be analyzed in nonviable (permeabilized) cells. Here, we show that CD134 (OX40) can be used as a discriminatory marker combined with CD25 to isolate and characterize viable CD4+ effector cells and Tregs. Before and during adjuvant arthritis in rats, coexpression of CD134 and CD25 identified activated Tregs consistently, as these T cells proliferated poorly to disease-associated antigens and were suppressive in vitro and in vivo. Depending on the time of isolation and location, CD4+ T cell populations expressing CD134 or CD25 contained effector/memory T cells. Analysis of the function, phenotype, and amount of the CD4+ T cell subsets in different lymph node stations revealed spatiotemporal differences in effector cell and Treg compartments during experimental arthritis.

Key Words: FoxP3 • immune regulation • kinetics • lymph nodes






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