Accuri C6 Flow Cytometer System
A more recent version of this article appeared on December 1, 2009

Published online before print September 16, 2009
This Article
Right arrow Full Text (Reprint (PDF))
Right arrow All Versions of this Article:
jlb.0509347v1
86/6/1359    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Megjugorac, N. J.
Right arrow Articles by Gallagher, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Megjugorac, N. J.
Right arrow Articles by Gallagher, G.
© by The Society for Leukocyte Biology
Journal of Leukocyte Biology, doi:10.1189/jlb.0509347

Received for publication May 22, 2009.
Revised July 15, 2009.
Accepted for publication August 18, 2009.

Article

Modulation of human plasmacytoid DC function by IFN-{lambda}1 (IL-29)

Nicholas J. Megjugorac , Grant E. Gallagher , and Grant Gallagher @

Genetic Immunology Laboratory, HUMIGEN LLC, the Institute for Genetic Immunology, Hamilton, New Jersey, USA

@ To whom correspondence should be addressed. E-mail: g.gallagher{at}humigen.org.


arrow
Abstract

The type III family of IFNs displays immunomodulatory and antiviral activity. Each member (IFN-{lambda}1, -2, and -3) signals through the same heterodimeric receptor complex, which consists of the binding and signaling subunit (IL-28R{alpha}) plus the IL-10R{beta} chain. Although the receptor has a wide tissue distribution, the direct effects of IFN-{lambda} on various immune cell subsets have not been fully characterized. We have identified high levels of IL-28R{alpha} mRNA in pDC from peripheral blood and hypothesized that IFN-{lambda} plays an important role in pDC maturation and development. We show that stimulation of pDC with HSV or Imiquimod causes an increase in IL-28R{alpha} mRNA. In these cells, IFN-{lambda}1 alters expression of the costimulatory molecules CD80 and ICOS-L and synergizes with IFN-{alpha} to up-regulate CD83. In addition, IFN-{lambda}1 has a variable effect on the homing molecule expression of pDC and mDC. IFN-{lambda}1-treated pDC display a marked difference in their ability to stimulate production of the signature cytokines IL-13, IFN-{gamma}, and IL-10 in a MLR. This work characterizes the variable effects of IFN-{lambda} on DC surface molecule expression and identifies a role in pDC activation and immunostimulatory potential.

Key Words: mDC • ICOS-L • T regulatory cell