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A more recent version of this article appeared on December 1, 2004

Published online before print August 31, 2004
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© by The Society for Leukocyte Biology
Journal of Leukocyte Biology, doi:10.1189/jlb.0404260

Received for publication April 30, 2004.
Revised July 12, 2004.
Accepted for publication July 20, 2004.

Article

Membrane retrieval in neutrophils during phagocytosis: inhibition by M protein-expressing S. pyogenes bacteria

Susanne Bauer and Hans Tapper @

Department of Cell and Molecular Biology, Section for Molecular Pathogenesis, BMC, B14, Lund University, Sweden

@ To whom correspondence should be addressed. E-mail: hans.tapper{at}medkem.lu.se.


  Abstract

During phagocytosis and phagosome maturation, complex membrane traffic events must be coordinated. We have observed, using fluorescent fluid-phase and membrane markers, that in the human neutrophil, internalization of nonopsonized, Gram-positive bacteria, but not of latex beads, is accompanied by a rapid and localized formation of pinosomal structures. This pinocytic response is calcium-dependent but insensitive to actin cytoskeleton disruption and wortmannin treatment. Contrary to what we observe, endosomal structures usually are considered to participate in phagosome formation by providing necessary membrane to form phagosomes. Instead, our results show a coupling between neutrophil secretory and membrane-retrieval processes during phagosome maturation, and we suggest that the observed, localized pinocytic response is linked to the secretion of azurophilic granules toward nascent phagosomes. Accordingly, M and M-like protein-expressing Streptococcus pyogenes bacteria, which are able to survive inside neutrophil phagosomes, inhibit the secretion of azurophilic granules to phagosomes and pinosome formation.

Key Words: membrane traffic • pinocytosis • secretion • localization • azurophilic granules




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 Infect. Immun.Home page
H. A. Phelps and M. N. Neely
SalY of the Streptococcus pyogenes Lantibiotic Locus Is Required for Full Virulence and Intracellular Survival in Macrophages
Infect. Immun., September 1, 2007; 75(9): 4541 - 4551.
[Abstract] [Full Text] [PDF]


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