Accuri C6 Flow Cytometer System
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Published online before print November 16, 2006
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© by The Society for Leukocyte Biology
Journal of Leukocyte Biology, doi:10.1189/jlb.0306229

Received for publication March 30, 2006.
Revised October 24, 2006.
Accepted for publication October 26, 2006.

Article

Calcitonin gene-related peptide (CGRP) inhibits early B cell development in vivo

Jerome J. Schlomer , Benjamin B. Storey , Radu-Tudor Ciornei , and Joseph P. McGillis @

Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky College of Medicine, Lexington, Kentucky, USA

@ To whom correspondence should be addressed. E-mail: jpmcgi01{at}uky.edu.


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Abstract

Recent in vitro studies suggest that calcitonin gene-related peptide (CGRP) inhibits early B cell differentiation; however, there is no evidence in the intact animal for a role for CGRP in B cell development. Here, we show that in vivo treatment of mice with CGRP reduces the number of IL-7 response B cell progenitors in bone marrow. A single CGRP treatment reduces IL-7-responsive B cell progenitors by up to 40% for up to 72 h. The reduction is dose-dependent and can be blocked by a CGRP receptor antagonist, CGRP8-37. CGRP, in serum following injection, is highly elevated at 30 min but returns to basal levels by 4 h, suggesting that a single injection of CGRP has long-lasting effects on B cell development. This report provides the first direct in vivo evidence that CGRP, a neuropeptide with multiple effects on mature lymphocytes, also plays a regulatory role in early B cell development in the bone marrow.

Key Words: neuropeptides • lymphopoiesis • hematopoiesis • neuroimmunology