Published online before print May 22, 2003
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2 integrins (CD11/CD18) in human polymorphonuclear neutrophils
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*Department of Physiology, Ludwig-Maximilians-Universität München, Germany; and Department of Physiology, Freie Universität Berlin, Germany
@ To whom correspondence should be addressed. E-mail: walzog{at}lrz.uni-muenchen.de.
A fine control of 
2 integrin (CD11/CD18)-mediated firm adhesion of human neutrophils to the endothelial cell monolayer is required to allow ordered emigration. To elucidate the molecular mechanisms that control this process, intracellular protein tyrosine signaling subsequent to 2 integrin-mediated ligand binding was studied by immunoprecipitation and Western blotting techniques. The 72-kDa Syk-kinase, which was tyrosine-phosphorylated upon adhesion, was found to coprecipitate with CD18, the -subunit of the 2 integrins. Moreover, inhibition of Syk-kinase by piceatannol enhanced adhesion and spreading but diminished N-formyl-Met-Leu-Phe-induced chemotactic migration. The enhancement of adhesiveness was associated with integrin clustering, which results in increased integrin avidity. In contrast, piceatannol had no effect on the surface expression or on the affinity of 2 integrins. Altogether, this suggests that Syk-kinase controls alternation of 2 integrin-mediated ligand binding with integrin detachment, which is necessary for neutrophil extravasation by regulating integrin clustering.
Key Words: LFA-1 • ICAM-1 • SDS-PAGE
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