Journal of Leukocyte Biology
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Originally published online as doi:10.1189/jlb.0606414 on November 29, 2006

Published online before print November 29, 2006
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(Journal of Leukocyte Biology. 2007;81:729-740.)
© 2007 by Society for Leukocyte Biology

Relevance of DC-SIGN in DC-induced T cell proliferation

Karlijn Gijzen, Paul J. Tacken, Aukje Zimmerman, Ben Joosten, I. Jolanda M. de Vries, Carl G. Figdor and Ruurd Torensma1

Department of Tumor Immunology, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands

1 Correspondence: Department of Tumor Immunology, NCMLS 278, Radboud University Nijmegen Medical Centre, Geert Grooteplein 26/28, 6525 GA Nijmegen, The Netherlands. E-mail: r.torensma{at}ncmls.ru.nl

The role of dendritic cell-specific ICAM-3-grabbing nonintegrin (DC-SIGN) in DC-T cell communication was assessed by analyzing the effect of DC-SIGN-blocking mAb in MLR. The results show that the degree of inhibition by DC-SIGN and LFA-1 mAb depends on the magnitude of the MLR and the maturation status of the DC. Addition of DC-SIGN mAb at several time-points during MLR showed that DC-SIGN is involved early on in DC-T cell contacts. This initial role is masked by strong adhesive and costimulatory mechanisms, indicating a short-lived effect of DC-SIGN in DC-T cell interactions. To examine this concept in more detail, the percentage of PBL capable of binding DC-SIGN was determined. Analysis of several donors revealed that 1–20% PBL bind to beads coated with recombinant DC-SIGN, and the DC-SIGN-binding cells comprised all major cell subsets found in blood. PBL isolated from a donor with high DC-SIGN-binding capacity were more prone to blocking by DC-SIGN mAb in MLR than PBL from a donor with low DC-SIGN-binding capacity. This study indicates an initial and transient role for DC-SIGN in T cell proliferation, which becomes apparent when T cell proliferation is low and when the percentage of DC-SIGN binding PBL is high.

Key Words: C-type lectins • integrins • mixed leukocyte reaction • conjugates • subsets




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