Journal of Leukocyte Biology Myeloid cells, immune suppression, tumor immunology
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Originally published online as doi:10.1189/jlb.0106011 on September 25, 2006

Published online before print September 25, 2006
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(Journal of Leukocyte Biology. 2006;80:1328-1336.)
© 2006 by Society for Leukocyte Biology

Human monocyte-derived dendritic cells express TLR9 and react directly to the CpG-A oligonucleotide D19

Victoria Hoene1, Matthias Peiser1 and Reinhard Wanner2

Institute of Molecular Biology and Bioinformatics, Charité-CBF, Berlin, Germany

2 Correspondence: Institute of Molecular Biology and Bioinformatics, Charité-CBF, Arnimallee 22, 14195 Berlin, Germany. E-mail: reinhard.wanner@charite.de

Oligodeoxynucleotides (ODNs) containing unmethylated CpG exhibit their immunostimulatory activities by binding to TLR. Here, we show that human monocyte-derived dendritic cells (moDC) contain TLR9 protein, surprisingly, in amounts comparable with plasmacytoid DC (pDC). Immature moDC but not mature moDC nor monocytes captured CpG-ODNs. moDC stimulation with the CpG-A ODN D19 up-regulated CD83, CD86, and HLA-DR. Without CD40 ligand costimulation, full maturation was not achieved. D19-stimulated moDC primed allogeneic CD4+-T cells for proliferation and differentiation into IFN-{gamma}-secreting Th1 cells. Neither IL-12 nor IL-6 or TNF-{alpha} was involved. Microarray analysis pointed to a participation of Type I IFNs. In fact, D19-stimulated moDC secreted considerable amounts of IFN-{alpha}. This indicates that moDC themselves sense viral and bacterial DNA and do not need help from pDC.

Key Words: cell surface molecules • cell activation • vaccination




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