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Published online before print February 6, 2007

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(Journal of Leukocyte Biology. 2007;81:1159.)
© 2007 by Society for Leukocyte Biology

C5a receptor expression in T lymphocytes

Department of Immunology, Georg-August-University Gottingen, Gottingen, Germany

¹ Correspondence: Department of Immunology, Georg-August-University Gottingen, Humboldtallee 32, D-37073 Gottingen, Germany. E-mail: jzwirne{at}gwdg.de

Key Words: anaphylatoxin • inflammation • receptor

Connelly et al. [¹ ] have published in a recent paper that C5a receptor expression in murine T lymphocytes is inducible under certain experimental conditions. Their conclusions rely exclusively on immunophenotyping by flow cytometry using a single mAb (20/70). In Acknowledgments, it is declared that "We are grateful to Dr. Jörg Zwirner (Georg-August-University Gottingen) for the mAb against C5a receptor."

I had originally provided mAb 20/70 against murine C5a receptor to the senior author of that paper, Dr. Jagannath, on a collaborative basis. However, I felt obliged to suggest withdrawal of coauthorship in the genuine belief that more experiments were required to verify expression of a functional C5a receptor on T lymphocytes. Specifically, I had asked for: 1) investigation of antibody binding after preincubation of lymphocytes with C5a, as reduced 20/70 binding might be expected through ligand-specific receptor blockade and/or internalization; and 2) investigation of C5a-induced calcium fluxes in C5a receptor-expressing T lymphocytes, as C3a-induced calcium fluxes in activated T cells have been observed previously [² ].

Unfortunately, Dr. Jagannath felt unable to perform these experiments.

Received November 22, 2006; accepted December 7, 2006.

REFERENCES

1. Connelly, M. A., Moulton, R. A., Smith, A. K., Lindsey, D. R., Sinha, M., Wetsel, R. A., Jagannath, C. (2007) Mycobacteria-primed macrophages and dendritic cells induce an up-regulation of complement C5a anaphylatoxin receptor (CD88) in CD3+ murine T cells J. Leukoc. Biol. 81,212-220[Abstract/Free Full Text]
2. Werfel, T., Kirchhoff, K., Wittmann, M., Begemann, G., Kapp, A., Heidenreich, F., Gotze, O., Zwirner, J. (2000) Activated human T lymphocytes express a functional C3a receptor J. Immunol. 165,6599-6605[Abstract/Free Full Text]

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This Article Full Text (PDF) All Versions of this Article: jlb.1106691v1 81/5/1159    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Zwirner, J. Search for Related Content PubMed PubMed Citation Articles by Zwirner, J.