HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

Published online before print May 8, 2008

This Article Full Text (Reprint (PDF)) Supplemental Figures and Tables All Versions of this Article: jlb.1207816v1 84/2/420    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Hernandez-Novoa, B. Articles by Kovacs, J. A. Search for Related Content PubMed PubMed Citation Articles by Hernandez-Novoa, B. Articles by Kovacs, J. A.

© by The Society for Leukocyte Biology
Journal of Leukocyte Biology, doi:10.1189/jlb.1207816

Received for publication December 7, 2007.
Revised April 9, 2008.
Accepted for publication April 11, 2008.

Article

Immune responses to Pneumocystis murina are robust in healthy mice but largely absent in CD40 ligand-deficient mice

Beatriz Hernandez-Novoa ^*, Lisa Bishop ^*, Carolea Logun ^*, Peter J. Munson , Eldad Elnekave , Zoila G. Rangel , Jennifer Barb , Robert L. Danner ^*, and Joseph A. Kovacs ^*@

*Critical Care Medicine Department, National Institutes of Health Clinical Center, Mathematical and Statistical Computing Laboratory, Division of Computational Bioscience, Center for Information Technology, and Laboratory of Parasitic Disease, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA

^@ To whom correspondence should be addressed. E-mail: jkovacs{at}nih.gov.

	Abstract

Pneumocystis is a pathogen of immunocompromised hosts but can also infect healthy hosts, in whom infection is rapidly controlled and cleared. Microarray methods were used to examine differential gene expression in the lungs of C57BL/6 and CD40 ligand knockout (CD40L-KO) mice over time following exposure to Pneumocystis murina. Immunocompetent C57BL/6 mice, which control and clear infection efficiently, showed a robust response to infection characterized by the up-regulation of 349 primarily immune response-associated genes. Temporal changes in the expression of these genes identified an early (Week 2), primarily innate response, which waned before the infection was controlled; this was followed by primarily adaptive immune responses that peaked at Week 5, which coincided with clearance of the infection. In conjunction with the latter, there was an increased expression of B cell-associated (Ig) genes at Week 6 that persisted through 11 weeks. In contrast, CD40L-KO mice, which are highly susceptible to developing severe Pneumocystis pneumonia, showed essentially no up-regulation of immune response-associated genes at Days 35–75. Immunohistochemical staining supported these observations by demonstrating an increase in CD4+, CD68+, and CD19+ cells in C57BL/6 but not CD40L-KO mice. Thus, the healthy host demonstrates a robust, biphasic response to infection by Pneumocystis; CD40L is an essential upstream regulator of the adaptive immune responses that efficiently control infection and prevent development of progressive pneumonia.

Key Words: immunodeficiency diseases • rodent • lung