Journal of Leukocyte Biology Myeloid cells, immune suppression, tumor immunology
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A more recent version of this article appeared on July 1, 2007

Published online before print April 17, 2007
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© by The Society for Leukocyte Biology
Journal of Leukocyte Biology, doi:10.1189/jlb.1106709

Received for publication November 30, 2006.
Revised February 28, 2007.
Accepted for publication March 19, 2007.

Article

Transcriptional response of human dendritic cells to Borrelia garinii--defective CD38 and CCR7 expression detected

Pauliina Hartiala *{dagger}@, Jukka Hytönen *, Jenni Pelkonen *, Katja Kimppa {ddagger}, Anne West {ddagger}, Markus A. Penttinen *, Juha Suhonen *{sect}, Riitta Lahesmaa {ddagger}, and Matti K. Viljanen *

*Department of Medical Microbiology and {dagger}Turku Graduate School of Biomedical Sciences, University of Turku, Finland; {ddagger}Turku Centre for Biotechnology, Turku, Finland; and {sect}Department of Medicine, Helsinki University Central Hospital, Helsinki, Finland

@ To whom correspondence should be addressed. E-mail: pauliina.hartiala{at}utu.fi.


  Abstract

Lyme borreliosis is a disease, which can affect several organs and cause a variety of symptoms. In some patients, the infection may become chronic, even after antibiotic therapy, and cause persisting damage. Dendritic cells (DC) are involved in the initiation of innate and adaptive immune responses. To study interactions between Borrelia garinii (Bg), one of the causative agents of Lyme borreliosis, and human DC, we used a cDNA microarray to compare the Bg-induced DC transcriptional response with the response induced by LPS. The Bg-induced response consisted of a smaller number of genes than the LPS-induced response. The microarray showed that the ectoenzyme CD38, which has an important role in DC chemotaxis and migration to lymph nodes, was strongly up-regulated by LPS but practically not at all by Bg. This finding was confirmed with quantitative RT-PCR and with flow cytometry at the protein level. In addition, RT-PCR showed that CCR7 expression was 11-fold greater in LPS-stimulated than in Bg-stimulated cells. These findings suggest that Bg may affect crucial DC functions by blocking the up-regulation of important molecules in DC migration to lymph nodes, thus affecting further immune responses in Lyme borreliosis infection.

Key Words: gene expression • Lyme borreliosis






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