| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
Published online before print June 16, 2005
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Article |
@,,
@
Departments of *Surgery, Immunology, and Medicine, University of Pittsburgh School of Medicine, Pennsylvania
@ To whom correspondence should be addressed. E-mail: haririmf{at}upmc.edu.
 |
Abstract |
|---|
Dendritic cells (DCs) activate T cells and regulate their differentiation into T helper cell type 1 (Th1) and/or Th2 cells. To identify DCs with differing abilities to direct Th1/Th2 cell differentiation, we cultured mouse bone marrow progenitors in granulocyte macrophage-colony stimulating factor (GM), GM + interleukin (IL)-4, or GM + IL-15 and generated three distinct DC populations. The GM + IL-4 DCs expressed high levels of CD80/CD86 and major histocompatibility complex (MHC) class II and produced low levels of IL-12p70. GM and GM + IL-15 DCs expressed low levels of CD80/CD86 and MHC class II. The GM + IL-15 DCs produced high levels of IL-12p70 and interferon (IFN)-
, whereas GM DCs produced only high levels of IL-12p70. Naive T cells stimulated with GM + IL-4 DCs secreted high levels of IL-4 and IL-5 in addition to IFN-. In contrast, the GM + IL-15 DCs induced higher IFN- production by T cells with little or no Th2 cytokines. GM DCs did not induce T cell polarization, despite producing large amounts of IL-12p70 following activation. A similar pattern of T cell activation was observed after in vivo administration of DCs. These data suggest that IL-12p70 production alone, although necessary for Th1 differentiation, is not sufficient to induce Th1 responses. These studies have implications for the use of DC-based vaccines in immunotherapy of cancer and other clinical conditions.
Key Words:
T cell activation • costimulation • IL-12p70 • IFN-
This article has been cited by other articles:
|
|
 |
|
  N. Sachdeva, V. Asthana, T. H. Brewer, D. Garcia, and D. Asthana Impaired Restoration of Plasmacytoid Dendritic Cells in HIV-1-Infected Patients with Poor CD4 T Cell Reconstitution Is Associated with Decrease in Capacity to Produce IFN-{alpha} but Not Proinflammatory Cytokines J. Immunol., August 15, 2008; 181(4): 2887 - 2897. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. Moulton, M. A. Mashruwala, A. K. Smith, D. R. Lindsey, R. A. Wetsel, D. L. Haviland, R. L. Hunter, and C. Jagannath Complement C5a anaphylatoxin is an innate determinant of dendritic cell-induced Th1 immunity to Mycobacterium bovis BCG infection in mice J. Leukoc. Biol., October 1, 2007; 82(4): 956 - 967. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Touitou, C. Daussy, B. Bodaghi, S. Camelo, Y. de Kozak, P. Lehoang, M.-C. Naud, A. Varin, B. Thillaye-Goldenberg, H. Merle-Beral, et al. Impaired Th1/Tc1 Cytokine Production of Tumor-Infiltrating Lymphocytes in a Model of Primary Intraocular B-Cell Lymphoma Invest. Ophthalmol. Vis. Sci., July 1, 2007; 48(7): 3223 - 3229. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S. Turner, P. A. Cohen, and O. J. Finn Lack of Effective MUC1 Tumor Antigen-Specific Immunity in MUC1-Transgenic Mice Results from a Th/T Regulatory Cell Imbalance That Can Be Corrected by Adoptive Transfer of Wild-Type Th Cells J. Immunol., March 1, 2007; 178(5): 2787 - 2793. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. L. Combe, M. M. Moretto, J. D. Schwartzman, J. P. Gigley, D. J. Bzik, and I. A. Khan Lack of IL-15 results in the suboptimal priming of CD4+ T cell response against an intracellular parasite PNAS, April 25, 2006; 103(17): 6635 - 6640. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Hokey, A. T. Larregina, G. Erdos, S. C. Watkins, and L. D. Falo Jr. Tumor Cell Loaded Type-1 Polarized Dendritic Cells Induce Th1-Mediated Tumor Immunity Cancer Res., November 1, 2005; 65(21): 10059 - 10067. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
