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© by The Society for Leukocyte Biology
Journal of Leukocyte Biology, doi:10.1189/jlb.0907599

Received for publication September 4, 2007.
Revised March 9, 2008.
Accepted for publication April 9, 2008.

Article

Sialyl lewisx antigen-expressing human CD4+ T and CD8+ T cells as initial immune responders in memory phenotype subsets

Yue Zhang *, Takayuki Ohkuri {dagger}, Daiko Wakita {dagger}, Yoshinori Narita {dagger}, Kenji Chamoto {dagger}, Hidemitsu Kitamura {dagger}, and Takashi Nishimura *{dagger}@

Divisions of *ROYCE’ Health Bioscience and {dagger}Immunoregulation, Section of Disease Control, Institute for Genetic Medicine, Hokkaido University, Sapporo, Japan

@ To whom correspondence should be addressed. E-mail: tak24{at}igm.hokudai.ac.jp.


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Abstract

Cytokine production by memory T cells in secondary immune responses has a critical role in host defenses. Previously, we had demonstrated that a unique antigen composed of sialyl lewisx (sLex) was expressed on CD45RO+ memory-phenotype subsets of human T cells. Here, we found that the sLex antigen was up-regulated on CD45RA+ naïve human CD4+ T and CD8+ T cells by TCR stimulation. In addition, sLex antigen-expressing CD4+ T and CD8+ T cells in human PBMCs were activated immediately by cytokine stimulations composed of IL-2 plus IL-12 or IL-15 in an antigen-independent manner. Moreover, the sLex-positive human CD8+ T cells significantly enhanced reverse antibody-dependent cellular cytotoxicity compared with a sLex-negative population. These findings clearly indicate that sLex antigen-expressing memory phenotype CD4+ T and CD8+ T cells contribute to early-stage immunity by providing a source of IFN-{gamma} and cytotoxicity, suggesting that they would be a key immunomodulator in host defenses.

Key Words: selectin • memory CD8 T cells • IL-15