Accuri C6 Flow Cytometer System
A more recent version of this article appeared on December 1, 2003

Published online before print November 11, 2003
This Article
Right arrow Full Text (Reprint (PDF))
Right arrow All Versions of this Article:
jlb.0803363v1
74/6/1139    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mohamedali, A.
Right arrow Articles by Thomas, N. S. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mohamedali, A.
Right arrow Articles by Thomas, N. S. B.
© by The Society for Leukocyte Biology
Journal of Leukocyte Biology, doi:10.1189/jlb.0803363

Received for publication August 1, 2003.
Revised August 22, 2003.
Accepted for publication September 9, 2003.

Article

Cyclin D2 controls B cell progenitor numbers

Azim Mohamedali *, Inês Soeiro {dagger}, Nicholas C. Lea *, Janet Glassford {dagger}, Lolita Banerji {dagger}, Ghulam J. Mufti *, Eric W.-F. Lam {dagger}, and N. Shaun B. Thomas *@

*Department of Haematological Medicine, Leukaemia Sciences, Guy’s, King’s, St. Thomas’ School of Medicine, Rayne Institute, London, United Kingdom; and {dagger}Cancer Research-UK Labs and Section of Cancer Cell Biology, Imperial College School of Medicine at Hammersmith Hospital, London, United Kingdom

@ To whom correspondence should be addressed. E-mail: nicholas.s.thomas{at}kcl.ac.uk.


arrow
Abstract

Cyclin D2 affects B cell proliferation and differentiation in vivo. It is rate-limiting for B cell receptor (BCR)-dependent proliferation of B cells, and cyclin D2-/- mice lack CD5+(B1) B lymphocytes. We show here that the bone marrow (BM) of cyclin D2-/- mice contains half the numbers of Sca1+B220+ B cell progenitors but normal levels of Sca1+ progenitor cells of other lineages. In addition, clonal analysis of BM from the cyclin D2-/- and cyclin D2+/+ mice confirmed that there were fewer B cell progenitors (B220+) in the cyclin D2-/- mice. In addition, the colonies from cyclin D2-/- mice were less mature (CD19lo) than those from cyclin D2+/+ mice (CD19Hi). The number of mature B2 B cells in vivo is the same in cyclin D2-/- and cyclin D2+/+ animals. Lack of cyclin D2 protein may be compensated by cyclin D3, as cyclin-dependent kinase (cdk)6 coimmunoprecipitates with cyclin D3 but not cyclin D1 from BM mononuclear cells of cyclin D2-/- mice. It is active, as endogenous retinoblastoma protein is phosphorylated at the cdk6/4-cyclin D-specific sites, S807/811. We conclude that cyclin D2 is rate-limiting for the production of B lymphoid progenitor cells whose proliferation does not depend on BCR signaling.

Key Words: mouse • bone marrow • Sca1+ • B lymphocytes • cellular proliferation




This article has been cited by other articles:


Home page
 J. Immunol.Home page
J. Klaewsongkram, Y. Yang, S. Golech, J. Katz, K. H. Kaestner, and N.-p. Weng
Kruppel-Like Factor 4 Regulates B Cell Number and Activation-Induced B Cell Proliferation
J. Immunol., October 1, 2007; 179(7): 4679 - 4684.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
P.-A. Deglesne, N. Chevallier, R. Letestu, F. Baran-Marszak, T. Beitar, C. Salanoubat, L. Sanhes, J. Nataf, C. Roger, N. Varin-Blank, et al.
Survival response to B-cell receptor ligation is restricted to progressive chronic lymphocytic leukemia cells irrespective of zap70 expression.
Cancer Res., July 15, 2006; 66(14): 7158 - 7166.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
S. Glennie, I. Soeiro, P. J. Dyson, E. W.-F. Lam, and F. Dazzi
Bone marrow mesenchymal stem cells induce division arrest anergy of activated T cells
Blood, April 1, 2005; 105(7): 2821 - 2827.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. Fernandez de Mattos, A. Essafi, I. Soeiro, A. M. Pietersen, K. U. Birkenkamp, C. S. Edwards, A. Martino, B. H. Nelson, J. M. Francis, M. C. Jones, et al.
FoxO3a and BCR-ABL Regulate cyclin D2 Transcription through a STAT5/BCL6-Dependent Mechanism
Mol. Cell. Biol., November 15, 2004; 24(22): 10058 - 10071.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. C. Chiles
Regulation and Function of Cyclin D2 in B Lymphocyte Subsets
J. Immunol., September 1, 2004; 173(5): 2901 - 2907.
[Abstract] [Full Text] [PDF]