Published online before print June 16, 2003

This Article Full Text (Reprint (PDF)) All Versions of this Article: jlb.0801757v1 74/3/412    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Li, Q. Articles by Nishida, T. Search for Related Content PubMed PubMed Citation Articles by Li, Q. Articles by Nishida, T.

© 2003 by The Society for Leukocyte Biology
Journal of Leukocyte Biology, doi:10.1189/jlb.0801757

Received for publication January 16, 2003.
Revised April 25, 2003.
Accepted for publication April 28, 2003.

Article

Enhancement by neutrophils of collagen degradation by corneal fibroblasts

Qin Li ^*, Ken Fukuda , Ying Lu ^*, Yoshikuni Nakamura ^*, Tai-ichiro Chikama ^*, Naoki Kumagai ^*, and Teruo Nishida ^*@

Departments of *Biomolecular Recognition and Ophthalmology and Ocular Pathophysiology, Yamaguchi University School of Medicine, Ube City, Japan

^@ To whom correspondence should be addressed. E-mail: nishida1{at}yamaguchi-u.ac.jp.

Abstract

Activated corneal fibroblasts and infiltrated leukocytes are thought to contribute to corneal ulceration. The potential roles of neutrophil-fibroblast and cell-matrix interactions in the degradation of stromal collagen associated with corneal ulceration have now been investigated with the use of three-dimensional cultures of rabbit cells in collagen gels. Degradation of collagen fibrils during culture was measured by spectrophotometric determination of released hydroxyproline. Whereas corneal fibroblasts alone degraded collagen fibrils to a small extent, neutrophils did not. However, the addition of neutrophils or neutrophil-conditioned medium (CM) to cultures of corneal fibroblasts resulted in a marked increase in the amount of collagen degraded by the fibroblasts. The effect of CM from neutrophils cultured in collagen gels on collagen degradation by corneal fibroblasts was greater than that of medium conditioned by neutrophils in monolayer culture. Immunoblot as well as reverse transcription and real-time polymerase chain reaction analyses revealed that neutrophil-CM stimulated the synthesis of matrix metalloproteinase (MMP)-1 and MMP-3 by corneal fibroblasts. The stimulatory effect of neutrophils on collagen degradation by corneal fibroblasts was inhibited by the synthetic MMP inhibitor ilomastat and by interleukin-1 (IL-1) receptor antagonist. These results suggest that factors secreted by collagen-stimulated neutrophils augment collagen degradation by corneal fibroblasts through a stimulatory effect on MMP synthesis and that IL-1 released by neutrophils may contribute to this effect.

Key Words: corneal ulcer • collagen gel • IL-1 receptor antagonist • matrix metalloproteinase

This article has been cited by other articles:

				W.-Y. Quan, J.-A. Ko, R. Yanai, Y. Nakamura, and T. Nishida Integrin-mediated inhibition of interleukin-8 secretion from human neutrophils by collagen type I J. Leukoc. Biol., March 1, 2010; 87(3): 487 - 491. [Abstract] [Full Text] [PDF]

				Y. Kondo, K. Fukuda, T. Adachi, and T. Nishida Inhibition by a Selective I{kappa}B Kinase-2 Inhibitor of Interleukin-1-Induced Collagen Degradation by Corneal Fibroblasts in Three-Dimensional Culture Invest. Ophthalmol. Vis. Sci., November 1, 2008; 49(11): 4850 - 4857. [Abstract] [Full Text] [PDF]

				Y. Liu, K. Kimura, R. Yanai, T.-i. Chikama, and T. Nishida Cytokine, Chemokine, and Adhesion Molecule Expression Mediated by MAPKs in Human Corneal Fibroblasts Exposed to Poly(I:C) Invest. Ophthalmol. Vis. Sci., August 1, 2008; 49(8): 3336 - 3344. [Abstract] [Full Text] [PDF]

				M. Lin, P. Jackson, A. M. Tester, E. Diaconu, C. M. Overall, J. E. Blalock, and E. Pearlman Matrix Metalloproteinase-8 Facilitates Neutrophil Migration through the Corneal Stromal Matrix by Collagen Degradation and Production of the Chemotactic Peptide Pro-Gly-Pro Am. J. Pathol., July 1, 2008; 173(1): 144 - 153. [Abstract] [Full Text] [PDF]

				Y. Lu, Y. Liu, K. Fukuda, Y. Nakamura, N. Kumagai, and T. Nishida Inhibition by triptolide of chemokine, proinflammatory cytokine, and adhesion molecule expression induced by lipopolysaccharide in corneal fibroblasts. Invest. Ophthalmol. Vis. Sci., September 1, 2006; 47(9): 3796 - 3800. [Abstract] [Full Text] [PDF]

				K. Fukuda, N. Kumagai, K. Yamamoto, Y. Fujitsu, N. Chikamoto, and T. Nishida Potentiation of Lipopolysaccharide-Induced Chemokine and Adhesion Molecule Expression in Corneal Fibroblasts by Soluble CD14 or LPS-Binding Protein Invest. Ophthalmol. Vis. Sci., September 1, 2005; 46(9): 3095 - 3101. [Abstract] [Full Text] [PDF]

				Y. Lu, K. Fukuda, Y. Nakamura, K. Kimura, N. Kumagai, and T. Nishida Inhibitory Effect of Triptolide on Chemokine Expression Induced by Proinflammatory Cytokines in Human Corneal Fibroblasts Invest. Ophthalmol. Vis. Sci., July 1, 2005; 46(7): 2346 - 2352. [Abstract] [Full Text] [PDF]

				Y. Lu, K. Fukuda, Y. Liu, N. Kumagai, and T. Nishida Dexamethasone Inhibition of IL-1-Induced Collagen Degradation by Corneal Fibroblasts in Three-Dimensional Culture Invest. Ophthalmol. Vis. Sci., September 1, 2004; 45(9): 2998 - 3004. [Abstract] [Full Text] [PDF]

				Y. Lu, K. Fukuda, K. Seki, Y. Nakamura, N. Kumagai, and T. Nishida Inhibition by Triptolide of IL-1-Induced Collagen Degradation by Corneal Fibroblasts Invest. Ophthalmol. Vis. Sci., December 1, 2003; 44(12): 5082 - 5088. [Abstract] [Full Text] [PDF]