Pepro Tech
A more recent version of this article appeared on February 1, 2008

Published online before print October 26, 2007
This Article
Right arrow Full Text (Reprint (PDF))
Right arrow All Versions of this Article:
jlb.0707457v1
83/2/296    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Veckman, V.
Right arrow Articles by Julkunen, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Veckman, V.
Right arrow Articles by Julkunen, I.
© by The Society for Leukocyte Biology
Journal of Leukocyte Biology, doi:10.1189/jlb.0707457

Received for publication July 12, 2007.
Revised September 12, 2007.
Accepted for publication October 5, 2007.

Article

Streptococcus pyogenes activates human plasmacytoid and myeloid dendritic cells

Ville Veckman @ and Ilkka Julkunen

Department of Viral Diseases and Immunology, National Public Health Institute, Helsinki, Finland

@ To whom correspondence should be addressed. E-mail: ville.veckman{at}ktl.fi.


arrow
Abstract

Human peripheral blood contains two major dendritic cell (DC) populations, namely CD11c–CD123+ plasmacytoid DCs (PDCs) and CD11c+CD123– myeloid DCs (MDCs). Although the activation of these DC types by various TLR ligands has been relatively well-characterized, less is known about the ability of whole live bacteria to induce PDC and MDC activation. In the present report, we have compared the activation of human PDCs and MDCs in response to major human bacterial pathogen Streptococcus pyogenes (group A streptococci) and influenza A virus. S. pyogenes stimulation resulted in the maturation of both DC types, as evidenced by enhanced expression of costimulatory molecules and production of proinflammatory cytokines and chemokines. Furthermore, S. pyogenes-stimulated PDCs and MDCs activated naïve CD4+ T cells and enhanced their Th1 cytokine production. Influenza A virus infection induced rapid PDC activation, whereas MDCs were extremely sensitive to influenza A virus-induced cell death. The most significant differences between DC types were seen in the production of IL-10 and IL-12, which were only produced by S. pyogenes-stimulated MDCs. Although S. pyogenes was able to induce PDC activation, only influenza A virus infection resulted in detectable IFN-{alpha} production. Our results show that depending on the infecting microbe, the functions of PDCs and MDCs may be partially overlapping, suggesting a considerable flexibility of the human DC system.

Key Words: cytokines • bacteria • virus • innate immunity




This article has been cited by other articles:


Home page
 J. Immunol.Home page
N. Noske, U. Kammerer, M. Rohde, and S. Hammerschmidt
Pneumococcal Interaction with Human Dendritic Cells: Phagocytosis, Survival, and Induced Adaptive Immune Response Are Manipulated by PavA
J. Immunol., August 1, 2009; 183(3): 1952 - 1963.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
T. G. Loof, O. Goldmann, and E. Medina
Immune Recognition of Streptococcus pyogenes by Dendritic Cells
Infect. Immun., June 1, 2008; 76(6): 2785 - 2792.
[Abstract] [Full Text] [PDF]