Journal of Leukocyte Biology eBioscience full spectrum cell analysis
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on April 1, 2008

Published online before print January 11, 2008
This Article
Right arrow Full Text (Reprint (PDF))
Right arrow All Versions of this Article:
jlb.0607392v1
83/4/817    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wang, H.-B.
Right arrow Articles by Weller, P. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wang, H.-B.
Right arrow Articles by Weller, P. F.
Related Collections
Right arrowRelated Article
© by The Society for Leukocyte Biology
Journal of Leukocyte Biology, doi:10.1189/jlb.0607392

Received for publication June 12, 2007.
Revised December 10, 2007.
Accepted for publication December 13, 2007.

Article

Eosinophils mediate early alum adjuvant-elicited B cell priming and IgM production

Hai-Bin Wang and Peter F. Weller @

Department of Medicine, Harvard Medical School, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA

@ To whom correspondence should be addressed. E-mail: pweller{at}bidmc.harvard.edu.


  Abstract

Alum, aluminum-hydroxide-containing compounds, long used as adjuvants in human vaccinations, functions by ill-defined, immunostimulatory mechanisms. Antigen-free alum has been shown to act via a previously unidentified, splenic Gr1+, IL-4-expressing myeloid cell population to stimulate early B cell priming. We demonstrate that the alum-elicited and -activated splenic myeloid cells are IL-4-expressing eosinophils that function to prime B cell responses. Eosinophils are the principal Gr1+, IL-4+ cells in the spleens 6 days following i.p.-alum administration. Alum-elicited splenic B cell priming, as evidenced by MHC II cross-linking-mediated calcium mobilization developed in wild-type BALB/c mice, was absent in {Delta}dblGATA BALB/c eosinophil-deficient mice and could be reconstituted by adoptive eosinophil infusions into the eosinophil-deficient mice. Moreover, early antigen-specific IgM antibody responses in alum-antigen-immunized mice were impaired in eosinophil-deficient mice and were restored with adoptive transfers of eosinophils. Thus, eosinophils, leukocytes of the innate immune system that contain preformed cytokines, including IL-4, have novel, immunomodulatory roles in the initial priming of B cells elicited by the adjuvant alum and in the optimal early B cell generation of antigen-specific IgM.

Key Words: BALB/c • MHC II


Related Article

The immunobiology of eosinophils—it’s a whole new world out there: an interview with Dr. Peter F. Weller
Helene F. Rosenberg
J. Leukoc. Biol. 2008 83: 822-823. [Full Text] [PDF]



This article has been cited by other articles:


Home page
 J. Immunol.Home page
H. Li, S. B. Willingham, J. P.-Y. Ting, and F. Re
Cutting Edge: Inflammasome Activation by Alum and Alum's Adjuvant Effect Are Mediated by NLRP3
J. Immunol., July 1, 2008; 181(1): 17 - 21.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Copyright © 2008 by the Society for Leukocyte Biology.