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Published online before print September 25, 2007

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© by The Society for Leukocyte Biology
Journal of Leukocyte Biology, doi:10.1189/jlb.0607348

Received for publication June 5, 2007.
Revised August 1, 2007.
Accepted for publication August 24, 2007.

Article

Aberrant dendritic cell differentiation initiated by the Mll-Een fusion gene does not require leukemic transformation

Qian Sun ^*, C. T. Kong ^*, F. P. Huang ^*, and L. C. Chan ^*@

Departments of *Pathology, S. H. Ho Foundation Research Laboratories, and Biochemistry, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China

^@ To whom correspondence should be addressed. E-mail: chanlc{at}pathology.hku.hk.

	Abstract

Dendritic cells (DCs), as specialized APCs, play a key role in the induction of anti-tumor immunity. They originate from bone marrow (BM) progenitors, which are frequently the targets of chromosomal translocations leading to development of leukemia. Aberrant DC differentiation and functions have been observed and are widely reported in patients with leukemia. It is not clear, however, whether such defects are a direct effect of a leukemic fusion gene or simply an outcome of the clinical disease. In this study, we demonstrate for the first time that knockin of the Mll-Een fusion gene can affect myeloid DC differentiation and functions directly, independent of the leukemic disease activities. We showed that the Mll-Een-expressing BM cells [enhanced green fluorescent protein+ (EGFP⁺)] from leukemic and nonleukemic mice had similarly impaired DC differentiation capacities with functional abnormalities. In contrast, BM cells without Mll-Een expression (EGFP^–) showed normal DC differentiation and functions. A reduction in the frequency of CD11c⁺ DCs was also observed within the EGFP⁺ population in spleen and lymph nodes, and these cells were dysfunctional. Taken together, our findings suggest that the Mll-Een fusion gene can affect myeloid DC differentiation directly and functions in a cell-autonomous manner, where fully leukemic transformation of the hematopoietic progenitors is not required exclusively. Therefore, the study provides evidence for a direct causal relationship between leukemic gene fusion and abnormal DC differentiation, possibly contributing to the development of leukemia.

Key Words: myeloid leukemia • EGFP