Accuri C6 Flow Cytometer System

Published online before print December 22, 2009
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© by The Society for Leukocyte Biology
Journal of Leukocyte Biology, doi:10.1189/jlb.0509359

Received for publication May 28, 2009.
Revised November 22, 2009.
Accepted for publication November 23, 2009.

Article

Macrophages from BALB/c and CBA/Ca mice differ in their cellular responses to Streptococcus pneumoniae

Vera M. Ripoll *@, Aras Kadioglu {dagger}, Roger Cox *, David A. Hume {ddagger}, and Paul Denny *

*Mammalian Genetics Unit, Medical Research Council, Harwell, United Kingdom;{dagger}Department of Infection, Immunity and Inflammation, University of Leicester, United Kingdom; and{ddagger}The Roslin Institute, University of Edinburgh, Scotland, United Kingdom

@ To whom correspondence should be addressed. E-mail: v.ripoll{at}har.mrc.ac.uk.


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Abstract

In a mouse model of pneumonia caused by Streptococcus pneumoniae, differences in the timing and vigor of the host inflammatory response have been associated with susceptibility to invasive disease. BALB/c and CBA/Ca mice are known to be resistant and susceptible to acute pneumococcal disease, respectively. In this study, we have demonstrated that BMM from BALB/c and CBA/Ca mice differ in their expression and regulation of TLR9 in response to S. pneumoniae. We have also shown that BMM from CBA/Ca mice failed to fully activate p38, NF-{kappa}B, and ERK 1/2 signaling pathways, resulting in reduced secretion of TNF-{alpha} and CCL5 in response to this pathogen. In addition, we have established that S. pneumoniae induced significant cell death in BMM from CBA/Ca mice. These findings indicate that variations between the two strains are likely to reflect differences in macrophage responses to the pathogen.

Key Words: mononuclear phagocytes • pneumococci • TLR9 • host pathogen interaction