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A more recent version of this article appeared on December 1, 2006

Published online before print September 8, 2006
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© by The Society for Leukocyte Biology
Journal of Leukocyte Biology, doi:10.1189/jlb.0506346

Received for publication May 23, 2006.
Revised July 3, 2006.
Accepted for publication August 14, 2006.

Article

Resolution of inflammation by retrograde chemotaxis of neutrophils in transgenic zebrafish

Jonathan R. Mathias *, Benjamin J. Perrin *, Ting-Xi Liu {dagger}, John Kanki {dagger}, A. Thomas Look {dagger}, and Anna Huttenlocher *@

*Department of Pharmacology, University of Wisconsin-Madison; and {dagger}Department of Pediatric Oncology, Dana-Farber Cancer Institute, Boston, Massachusetts

@ To whom correspondence should be addressed. E-mail: huttenlocher{at}wisc.edu.


  Abstract

Neutrophil chemotaxis to sites of inflammation is a critical process during normal immune responses to tissue injury and infection and pathological immune responses leading to chronic inflammation. Although progress has been made in understanding the mechanisms that promote neutrophil recruitment to inflamed tissue, the mechanisms that regulate the resolution phase of the inflammatory response have remained relatively elusive. To define the mechanisms that regulate neutrophil-mediated inflammation in vivo, we have developed a novel transgenic zebrafish in which the neutrophils express GFP under control of the myeloperoxidase promoter (zMPO:GFP). Tissue injury induces a robust, inflammatory response, which is characterized by the rapid chemotaxis of neutrophils to the wound site. In vivo time-lapse imaging shows that neutrophils subsequently display directed retrograde chemotaxis back toward the vasculature. These findings implicate retrograde chemotaxis as a novel mechanism that regulates the resolution phase of the inflammatory response. The zMPO:GFP zebrafish provides unique insight into the mechanisms of neutrophil-mediated inflammation and thereby offers opportunities to identify new regulators of the inflammatory response in vivo.

Key Words: GFP • leukocyte




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