Journal of Leukocyte Biology
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Published online before print November 15, 2006
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© by The Society for Leukocyte Biology
Journal of Leukocyte Biology, doi:10.1189/jlb.0406276

Received for publication April 15, 2006.
Revised October 18, 2006.
Accepted for publication October 20, 2006.

Article

The human EGF-TM7 receptor EMR3 is a marker for mature granulocytes

Mourad Matmati *, Walter Pouwels *, Robin van Bruggen {dagger}, Machiel Jansen {dagger}, Robert M. Hoek *, Arthur J. Verhoeven {dagger}, and Jörg Hamann *@

*Department of Experimental Immunology and {dagger}Sanquin Research and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands

@ To whom correspondence should be addressed. E-mail: j.hamann{at}amc.uva.nl.


  Abstract

EMR3 is a member of the epidermal growth factor-seven-transmembrane (EGF-TM7) family of adhesion class TM7 receptors. This family also comprises CD97, EMR1, EMR2, and EMR4. To characterize human EMR3 at the protein level, we generated Armenian hamster mAb. Using the mAb 3D7, we here demonstrate that EMR3, like other EGF-TM7 receptors, is expressed at the cell surface as a heterodimeric molecule consisting of a long extracellular {alpha}-chain, which possesses at its N terminus EGF-like domains and a membrane-spanning {beta}-chain. Flow cytometric analysis revealed that all types of myeloid cells express EMR3. In peripheral blood, the highest expression of EMR3 was found on granulocytes. More mature CD16+ monocytes express high levels of EMR3, and CD16- monocytes and blood dendritic cell (DC) antigen-1+ myeloid DC are EMR3dim/low. Lymphocytes and plasmacytoid DC are EMR3-. It is interesting that in contrast with CD97 and EMR2, CD34+CD33-/CD38--committed hematopoietic stem cells and CD34+CD33+/CD38+ progenitors in bone marrow do not express EMR3. In vitro differentiation of HL-60 cells and CD34+ progenitor cells revealed that EMR3 is only up-regulated during late granulopoiesis. These results demonstrate that the expression of EGF-TM7 receptors on myeloid cells is differentially regulated. EMR3 is the first family member found mainly on granulocytes.

Key Words: expression profiling • granulopoiesis • monoclonal antibody




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