Accuri C6 Flow Cytometer System
A more recent version of this article appeared on August 1, 2008

Published online before print May 21, 2008
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© by The Society for Leukocyte Biology
Journal of Leukocyte Biology, doi:10.1189/jlb.0308170

Received for publication March 11, 2008.
Revised April 15, 2008.
Accepted for publication April 24, 2008.

Article

Postnatal maturation of total cell content and up-regulated surface expression of Mac-1 (CD11b/CD18) in polymorphonuclear leukocytes of human infants

Shawn W. Storm *{dagger}, M. Michele Mariscalco {ddagger}, and Michael F. Tosi *{ddagger}@

*Department of Pediatrics, Case Western Reserve University, Cleveland, Ohio, USA; {dagger}Department of Biology, John Carroll University, Cleveland, Ohio, USA; and {ddagger}Section of Leukocyte Biology, Baylor College of Medicine, Houston, Texas, USA

@ To whom correspondence should be addressed. E-mail: michael.tosi{at}mssm.edu.


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Abstract

Markedly deficient expression of membrane-activated complex 1 (Mac-1; CD11b/CD18) by polymorphonuclear neutrophils (PMN) of human neonates compared with adults is well documented. To define postnatal maturation of Mac-1 expression of PMN, lysates of PMN from 21 infants, aged 1–14 months, and concurrent adult controls were assayed by ELISA for total cell content of Mac-1 and LFA-1 (CD11a/CD18), and LFA-1 content was within the normal adult range at all ages tested. Mac-1 content was ~50% of adult levels for infants 1–2 months of age and steadily increased to reach normal adult levels by 11–12 months of age. For a separate group of 25 infants, aged 0.5–11 months, measurement of surface expression of Mac-1 and LFA-1 on activated PMN by immunofluorescence flow cytometry yielded results that were similar to those obtained by ELISA.

Key Words: {beta}-2 integrins • children • development