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Published online before print January 30, 2007

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© by The Society for Leukocyte Biology
Journal of Leukocyte Biology, doi:10.1189/jlb.0306209

Received for publication March 15, 2006.
Revised December 5, 2006.
Accepted for publication December 23, 2006.

Article

Molecular mechanisms involved in interleukin-4 (IL-4)-induced human neutrophils: expression and regulation of suppressor of cytokine signaling (SOCS)

INRS-Institut Armand-Frappier, Université du Québec, Pointe-Claire, Canada

^@ To whom correspondence should be addressed. E-mail: denis.girard{at}iaf.inrs.ca.

	Abstract

IL-4 is a CD132-dependent cytokine known to activate the Jak-STAT pathway in different cells and cell lines. Although IL-4 has been demonstrated previously to be an agonist in human neutrophils, its capacity to activate different cell signaling pathways in these cells has never been investigated. Two types of IL-4 receptor (IL-4R) exist: the Type I (CD132/IL-4R heterodimer) and the Type II (IL-4R/IL-13R1 heterodimer). In a previous study, we demonstrated that neutrophils express the Type I receptor. Herein, using flow cytometry, we demonstrated that neutrophils, unlike U-937 cells, do not express IL-13R1 and IL-13R2 and confirmed the expression of CD132 and IL-4R on their surface. We also demonstrated that IL-4 induced phosphorylation of Syk, p38, Erk-1/2, JNK, Jak-1, Jak-2, STAT6, and STAT1 and that treatment of cells with the inhibitors piceatannol, SB203580, PD98059, or AG490 reversed the ability of IL-4 to delay neutrophil apoptosis. Using RT-PCR, we demonstrated for the first time that neutrophils express mRNA for all suppressor of cytokine signaling (SOCS) members, namely SOCS1-7 and cytokine-inducible Src homology 2 protein. It is interesting that IL-4 increased expression of SOCS3 at the mRNA and protein levels. The effect of IL-4 on SOCS3 protein expression was increased markedly when the proteasome inhibitor MG132 was added to the cultures, but this was inhibited by cycloheximide, suggesting that SOCS3 is de novo-synthesized in response to IL-4. We conclude that neutrophils express only the Type I IL-4R on their surface and that IL-4 signals via different cell signaling pathways, including the Jak/STAT/SOCS pathway.

Key Words: inflammation • cytokine • receptor • Jak • STAT • SOCS3

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