Accuri C6 Flow Cytometer System
A more recent version of this article appeared on September 1, 2005

Published online before print July 6, 2005
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© by The Society for Leukocyte Biology
Journal of Leukocyte Biology, doi:10.1189/jlb.0305167





Article

Morphine inhibits CD8+ T cell-mediated, noncytolytic, anti-HIV activity in latently infected immune cells

Xu Wang *, Ning Tan {dagger}, Steven D. Douglas *, Ting Zhang *, Yan-Jian Wang *, and Wen-Zhe Ho *@

*Division of Allergy and Immunology, Joseph Stokes, Jr., Research Institute at The Children’s Hospital of Philadelphia, Department of Pediatrics, University of Pennsylvania School of Medicine; and {dagger}Department of Medicine, The People’s Hospital of Guangdong Province, Guangzhou, People’s Republic of China

@ To whom correspondence should be addressed. E-mail: ho{at}email.chop.edu.


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Abstract

Opiates have profound effects on the function of human immune cells and are a possible cofactor in the immunopathogenesis of human immunodeficiency virus (HIV) disease. We investigated the impact of morphine on CD8+ T cell-mediated, noncytotoxic, anti-HIV activity in latently infected human immune cells. Morphine inhibited the noncytotoxic, anti-HIV activity of CD8+ T cells in HIV latently infected cells (U1 and J1.1). Naltrexone abrogated the morphine-mediated, inhibitory effect on the noncytotoxic, anti-HIV activity of CD8+ T cells. Interferon-{gamma} (IFN-{gamma}), a potent antiviral cytokine produced by CD8+ T cells, was partially responsible for CD8+ T cell-mediated, noncytotoxic, anti-HIV activity. The anti-HIV activity of IFN-{gamma} was also compromised by morphine treatment. Further, morphine attenuated CD8+ T cell-mediated suppression of the HIV long-terminal repeat promoter activation. Morphine also inhibited CD8+ T cell-induced expression of the signal transducer and activator of transcription-1, an important transcriptional factor in the IFN signaling pathway. These data provide additional evidence to support the notion that opioids play a role in impairing the anti-HIV function of the immune system.

Key Words: IFN-{gamma} • PBMC • U1 cells • J1.1 cells