Journal of Leukocyte Biology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on December 1, 2005

Published online before print October 4, 2005
This Article
Right arrow Full Text (Reprint (PDF))
Right arrow All Versions of this Article:
jlb.0304123v1
78/6/1185    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rahim, R. T.
Right arrow Articles by Eisenstein, T. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rahim, R. T.
Right arrow Articles by Eisenstein, T. K.
© by The Society for Leukocyte Biology
Journal of Leukocyte Biology, doi:10.1189/jlb.0304123

Received for publication March 3, 2004.
Revised August 22, 2005.
Accepted for publication August 30, 2005.

Article

Splenic macrophages and B cells mediate immunosuppression following abrupt withdrawal from morphine

Rahil T. Rahim *{dagger}, Joseph J. Meissler Jr. *{dagger}, Martin W. Adler {dagger}{ddagger}, and Toby K. Eisenstein *{dagger}@

Departments of *Microbiology and Immunology and {ddagger}Pharmacology and {dagger}Center for Substance Abuse Research, Temple University School of Medicine, Philadelphia, Pennsylvania

@ To whom correspondence should be addressed. E-mail: tke{at}temple.edu.


  Abstract

We have previously shown that abrupt withdrawal (AW) from morphine induces greater than 80% immunosuppression in murine spleen cells, as assessed by the capacity to mount an in vitro plaque-forming cell response to sheep red blood cells. Present studies about the mechanisms of immunosuppression following AW showed that addition of highly enriched (CD11b+) splenic macrophages (obtained by cell sorting or magnetic separation) from AW mice to cultures of normal, unfractionated spleen cells suppressed immune responses. Further, addition of highly enriched (CD19+) B cells (but not T cells) from AW mice to normal cells was also immunosuppressive. B cells from AW mice were also able to inhibit the proliferative response of normal spleen cells to concanavalin A but not to lipopolysaccharide. Overall, the data suggest that immunosuppression by AW spleen cells is a result of active suppression by macrophages and B cells.

Key Words: neuroimmunology • plaque-forming cell • Con A






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Copyright © 2005 by the Society for Leukocyte Biology.