Published online before print October 17, 2006

This Article Full Text (Reprint (PDF)) All Versions of this Article: jlb.0206124v1 81/1/328    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Li, J. Articles by Liles, W. C. Search for Related Content PubMed PubMed Citation Articles by Li, J. Articles by Liles, W. C.

© by The Society for Leukocyte Biology
Journal of Leukocyte Biology, doi:10.1189/jlb.0206124

Received for publication February 28, 2006.
Revised August 8, 2006.
Accepted for publication August 25, 2006.

Article

cDNA microarray analysis reveals fundamental differences in the expression profiles of primary human monocytes, monocyte-derived macrophages, and alveolar macrophages

Jiangning Li ^*, David K. Pritchard ^*@, Xi Wang ^*, David R. Park , Roger Bumgarner , Stephen M. Schwartz ^*, and W. Conrad Liles ^*

Departments of *Pathology, Medicine, and Microbiology, University of Washington, Seattle, Washington, USA

^@ To whom correspondence should be addressed. E-mail: dpritch{at}u.washington.edu.

Abstract

We report the systematic use of large-scale cDNA microarrays to study the gene expression profiles of primary human peripheral blood monocytes (MONO) in comparison with in vitro-differentiated, M-CSF-induced MONO-derived macrophages (MAC) and primary human alveolar MAC (AM), obtained by bronchoalveolar lavage from the lungs of normal volunteers. These studies revealed large-scale differences in the gene expression profile between both MAC types (MAC and AM) and MONO. In addition, large differences were observed in the gene expression profiles of the two MAC types. Specifically, 21% of genes on the array (2904 out of 13,582) were differentially expressed between AM and MONO, and 2229 out of 13,583 probes were differentially expressed between MAC and AM. Our expression data show remarkable differences in gene expression between different MAC subpopulations and emphasize the heterogeneity of different MAC populations. This study underscores the need to scrutinize models of MAC biology for relevance to specific disease processes.

Key Words: differentiation • heterogeneity • gene expression

This article has been cited by other articles:

				L. V. Deriy, E. A. Gomez, G. Zhang, D. W. Beacham, J. A. Hopson, A. J. Gallan, P. D. Shevchenko, V. P. Bindokas, and D. J. Nelson Disease-causing Mutations in the Cystic Fibrosis Transmembrane Conductance Regulator Determine the Functional Responses of Alveolar Macrophages J. Biol. Chem., December 18, 2009; 284(51): 35926 - 35938. [Abstract] [Full Text] [PDF]

				J. Li, J. S. Campbell, C. Mitchell, R. S. McMahan, X. Yu, K. J. Riehle, R. E. Bumgarner, and N. Fausto Relationships between Deficits in Tissue Mass and Transcriptional Programs after Partial Hepatectomy in Mice Am. J. Pathol., September 1, 2009; 175(3): 947 - 957. [Abstract] [Full Text] [PDF]

				K. M. Irvine, M. R. Andrews, M. A. Fernandez-Rojo, K. Schroder, C. J. Burns, S. Su, A. F. Wilks, R. G. Parton, D. A. Hume, and M. J. Sweet Colony-stimulating factor-1 (CSF-1) delivers a proatherogenic signal to human macrophages J. Leukoc. Biol., February 1, 2009; 85(2): 278 - 288. [Abstract] [Full Text] [PDF]

				E. J. Cornish, B. J. Hurtgen, K. McInnerney, N. L. Burritt, R. M. Taylor, J. N. Jarvis, S. Y. Wang, and J. B. Burritt Reduced Nicotinamide Adenine Dinucleotide Phosphate Oxidase-Independent Resistance to Aspergillus fumigatus in Alveolar Macrophages J. Immunol., May 15, 2008; 180(10): 6854 - 6867. [Abstract] [Full Text] [PDF]