Vancouver
A more recent version of this article appeared on September 1, 2005

Published online before print June 7, 2005
This Article
Right arrow Full Text (Reprint (PDF))
Right arrow All Versions of this Article:
jlb.0205063v1
78/3/705    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Echchannaoui, H.
Right arrow Articles by Landmann, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Echchannaoui, H.
Right arrow Articles by Landmann, R.
© by The Society for Leukocyte Biology
Journal of Leukocyte Biology, doi:10.1189/jlb.0205063

Received for publication February 1, 2005.
Revised April 28, 2005.
Accepted for publication April 29, 2005.

Article

CD14 deficiency leads to increased MIP-2 production, CXCR2 expression, neutrophil transmigration, and early death in pneumococcal infection

Hakim Echchannaoui *, Karl Frei {dagger}, Maryse Letiembre *, Robert M. Strieter {ddagger}, Yoshiyuki Adachi {sect}, and Regine Landmann *@

*Division of Infectious Diseases, Department of Research, University Hospitals, Basel, Switzerland; {dagger}Department of Neurosurgery, University Hospital, Zurich, Switzerland; {ddagger}Departments of Medicine and Pathology and Laboratory Medicine, David Geffen School of Medicine at University of California, Los Angeles; and {sect}Laboratory of Immunopharmacology of Microbial Products, Tokyo University of Pharmacy and Life Science, Japan

@ To whom correspondence should be addressed. E-mail: Regine.Landmann{at}unibas.ch.


arrow
Abstract

CD14 is a myeloid receptor for bacterial cell membrane/wall components, for which we previously showed a strong induction in cerebrospinal fluid (CSF) during meningitis. Here, we studied CD14 function in murine Streptococcus pneumoniae meningitis by using wild-type (WT), CD14-/- mice, and WT mice pretreated with neutralizing anti-CD14 antibodies. Early polymorphonuclear leukocytes (PMN) immigration was more pronounced in CSF of CD14-/- than of WT mice. This was not a result of altered adherence molecule expression in blood and CSF PMN or brain endothelial cells. Macrophage inflammatory protein-2 (MIP-2) and keratinocyte-derived chemokine levels were similar in CSF in both strains, but MIP-2 was higher in infected brain and in brain-derived endothelial cells infected in vitro in CD14-/- than in WT mice. CD14-/- PMN demonstrated increased expression of CXC chemokine receptor 2 (CXCR2) after infection and stronger in vitro chemotaxis than WT PMN toward CSF from WT or CD14-/- mice and toward MIP-2. Excess PMN migration in CD14-/- mice did not result in improved bacterial clearing but in increased tumor necrosis factor in CSF, higher disease severity, and earlier death. Pretreatment with anti-CXCR2 reduced PMN infiltration into CSF and brain MIP-2 production and abolished earlier mortality in CD14-/- mice. In conclusion, CD14 has a protective effect in pneumococcal meningitis by slowing PMN migration via MIP-2 and CXCR2 modulation.

Key Words: chemokines • polymorphonuclear cells • meningitis




This article has been cited by other articles:


Home page
 Infect. Immun.Home page
S. Ribes, S. Ebert, T. Regen, A. Agarwal, S. C. Tauber, D. Czesnik, A. Spreer, S. Bunkowski, H. Eiffert, U.-K. Hanisch, et al.
Toll-Like Receptor Stimulation Enhances Phagocytosis and Intracellular Killing of Nonencapsulated and Encapsulated Streptococcus pneumoniae by Murine Microglia
Infect. Immun., February 1, 2010; 78(2): 865 - 871.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. Schmaler, N. J. Jann, F. Ferracin, L. Z. Landolt, L. Biswas, F. Gotz, and R. Landmann
Lipoproteins in Staphylococcus aureus Mediate Inflammation by TLR2 and Iron-Dependent Growth In Vivo
J. Immunol., June 1, 2009; 182(11): 7110 - 7118.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
S. M. Farooq, R. Stillie, M. Svensson, C. Svanborg, R. M. Strieter, and A. W. Stadnyk
Therapeutic Effect of Blocking CXCR2 on Neutrophil Recruitment and Dextran Sodium Sulfate-Induced Colitis
J. Pharmacol. Exp. Ther., April 1, 2009; 329(1): 123 - 129.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. Mildner, M. Djukic, D. Garbe, A. Wellmer, W. A. Kuziel, M. Mack, R. Nau, and M. Prinz
Ly-6G+CCR2- Myeloid Cells Rather Than Ly-6ChighCCR2+ Monocytes Are Required for the Control of Bacterial Infection in the Central Nervous System
J. Immunol., August 15, 2008; 181(4): 2713 - 2722.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
N. Kesteman, G. Vansanten, B. Pajak, S. M. Goyert, and M. Moser
Injection of lipopolysaccharide induces the migration of splenic neutrophils to the T cell area of the white pulp: role of CD14 and CXC chemokines
J. Leukoc. Biol., March 1, 2008; 83(3): 640 - 647.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. C. Dessing, S. Knapp, S. Florquin, A. F. de Vos, and T. van der Poll
CD14 Facilitates Invasive Respiratory Tract Infection by Streptococcus pneumoniae
Am. J. Respir. Crit. Care Med., March 15, 2007; 175(6): 604 - 611.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
G. K. Paterson and T. J. Mitchell
Innate immunity and the pneumococcus
Microbiology, February 1, 2006; 152(2): 285 - 293.
[Abstract] [Full Text] [PDF]