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Published online before print September 15, 2004

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© by The Society for Leukocyte Biology
Journal of Leukocyte Biology, doi:10.1189/jlb.0204101

Received for publication February 20, 2004.
Revised July 21, 2004.
Accepted for publication August 24, 2004.

Article

Human dendritic cells, which differentially trigger IgA complex endocytosis, up-regulate two IgA receptors (CD89 and CD71) during maturation

Benoit Pasquier ^*, Yves Lepelletier , Cédric Baude , Olivier Hermine , and Renato C. Monteiro ^*@

*INSERM E0225, Bichat Medical School, Paris, France; and CNRS UMR8147, Necker Hospital, Paris, France

^@ To whom correspondence should be addressed. E-mail: MONTEIRO{at}BICHAT.INSERM.FR.

	Abstract

Dendritic cells (DC) are the most efficient antigen-presenting cells residing in mainly peripheral tissues. Antigen uptake by DC is particularly efficient, being mediated by various receptors such as lectin, scavenger receptors, and Fc receptors (FcRs). Immunoglobulin A (IgA) is part of the first-line immune barrier in mucosae, where DC are numerous. A member of the FcR family, FcRI, is expressed on interstitial DC. We report here that monocyte-derived DC (Mo-DC) express another IgA receptor (IgA-R), the transferrin receptor (TfR), even in the absence of DC proliferation in vitro. Upon incubation with inflammatory cytokines such as tumor necrosis factor and interleukin (IL)-1 or maturating agents (lipopolysaccharide, CD40 ligand), FcRI and TfR expression on Mo-DC was specifically up-regulated, whereas FcRs and FcRI expression was down-regulated. IgA-Rs were functional, being able to mediate endocytosis by immature and activated Mo-DC. Although FcRI internalized IgA complexes on both types of DC, TfR was only able to mediate IgA complex internalization by immature cells. Cross-linking of FcRI but not of TfR resulted in up-regulation of major histocompatibility complex (MHC) class II/CD86 expression and secretion of IL-10 and IL-12 by immature Mo-DC. Moreover, in activated Mo-DC, cross-linking of FcRI could up-regulated MHC class II/CD86 and triggered IL-10 secretion. Our findings led us to propose that FcRI expressed by interstitial-type DC could play a critical role to sample IgA-recognized antigens and also in DC activation.

Key Words: Fc receptor • transferrin receptor • dendritic cells