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A more recent version of this article appeared on September 1, 2007

Published online before print June 5, 2007
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© by The Society for Leukocyte Biology
Journal of Leukocyte Biology, doi:10.1189/jlb.0107050

Received for publication January 22, 2007.
Revised April 20, 2007.
Accepted for publication May 4, 2007.

Article

Fibrinogen is localized on dark zone follicular dendritic cells in vivo and enhances the proliferation and survival of a centroblastic cell line in vitro

Eric A. Lefevre *@, Wayne R. Hein {dagger}, Zania Stamataki *, Louise S. Brackenbury *, Emma A. Supple *, Lawrence G. Hunt *, Paul Monaghan {ddagger}, Gwenoline Borhis {sect}, Yolande Richard {sect}, and Bryan Charleston *

*Compton Laboratory, Institute for Animal Health, Compton, United Kingdom; {dagger}AgResearch Ltd., Wallaceville Animal Research Centre, Upper Hutt, New Zealand; {ddagger}Pirbright Laboratory, Institute for Animal Health, Pirbright, United Kingdom; and {sect}Service d’Immuno-Virologie, Commissariat à l’Energie Atomique, DSV/iMETI, Fontenay aux Roses, France

@ To whom correspondence should be addressed. E-mail: eric.lefevre{at}bbsrc.ac.uk.


  Abstract

Follicular dendritic cells (FDC) in the germinal centers (GC) of secondary lymphoid organs increase the survival and proliferation of antigen-stimulated B cells and are pivotal for the affinity maturation of an antibody response and for maintenance of B cell immunological memory. The dark zone (DZ) and the light zone (LZ) constitute distinct areas of the GC containing different subtypes of FDC as identified by their morphology and phenotype. Until now, most available FDC-specific reagents identify LZ FDC, and there are no reagents recognizing DZ FDC specifically. Here, we report a new mAb, D46, which stains FDC specifically in the DZ of bovine and ovine GC within the secondary follicles. We identify its ligand as bovine fibrinogen and using commercially available, anti-human fibrinogen antibodies, show that this inflammatory protein is also present on DZ FDC of human GC within palatine tonsils. In vitro, the addition of exogenous fibrinogen stimulates the proliferation and survival of BCR-stimulated L3055 cells, which constitute a clonal population of centroblastic cells and retain important features of normal GC B cells. Together, our results suggest that fibrinogen localized on DZ FDC could support the extensive proliferation and survival of GC B cells within the DZ in vivo.

Key Words: FDC subpopulations • B cells • secondary follicle • lymphoid organs






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Copyright © 2007 by the Society for Leukocyte Biology.