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A more recent version of this article appeared on December 1, 2006

Published online before print September 25, 2006
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© by The Society for Leukocyte Biology
Journal of Leukocyte Biology, doi:10.1189/jlb.0106011

Received for publication January 6, 2006.
Revised July 21, 2006.
Accepted for publication August 19, 2006.

Article

Human monocyte-derived dendritic cells express TLR9 and react directly to the CpG-A oligonucleotide D19

Victoria Hoene , Matthias Peiser , and Reinhard Wanner @

Institute of Molecular Biology and Bioinformatics, Charité-CBF, Berlin, Germany

@ To whom correspondence should be addressed. E-mail: reinhard.wanner{at}charite.de.


  Abstract

Oligodeoxynucleotides (ODNs) containing unmethylated CpG exhibit their immunostimulatory activities by binding to TLR. Here, we show that human monocyte-derived dendritic cells (moDC) contain TLR9 protein, surprisingly, in amounts comparable with plasmacytoid DC (pDC). Immature moDC but not mature moDC nor monocytes captured CpG-ODNs. moDC stimulation with the CpG-A ODN D19 up-regulated CD83, CD86, and HLA-DR. Without CD40 ligand costimulation, full maturation was not achieved. D19-stimulated moDC primed allogeneic CD4+-T cells for proliferation and differentiation into IFN-{gamma}-secreting Th1 cells. Neither IL-12 nor IL-6 or TNF-{alpha} was involved. Microarray analysis pointed to a participation of Type I IFNs. In fact, D19-stimulated moDC secreted considerable amounts of IFN-{alpha}. This indicates that moDC themselves sense viral and bacterial DNA and do not need help from pDC.

Key Words: cell surface molecules • cell activation • vaccination




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