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Published online before print May 22, 2003

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© 2003 by The Society for Leukocyte Biology
Journal of Leukocyte Biology, doi:10.1189/jlb.0103008

Received for publication January 9, 2003.
Revised March 6, 2003.
Accepted for publication March 24, 2003.

Article

RNA analysis of B cell lines arrested at defined stages of differentiation allows for an approximation of gene expression patterns during B cell development

Panagiotis Tsapogas ^*, Thomas Breslin , Sven Bilke , Anna Lagergren ^*, Robert Månsson ^*, David Liberg ^*, Carsten Peterson , and Mikael Sigvardsson ^*@

*Laboratory for Cellular Differentiation, Department for Stemcell Biology, and Department of Theoretical Physics, Lund University, Sweden

^@ To whom correspondence should be addressed. E-mail: Mikael.Sigvardsson{at}stemcell.lu.se.

	Abstract

The development of a mature B lymphocyte from a bone marrow stem cell is a highly ordered process involving stages with defined features and gene expression patterns. To obtain a deeper understanding of the molecular genetics of this process, we have performed RNA expression analysis of a set of mouse B lineage cell lines representing defined stages of B cell development using Affymetrix^TM microarrays. The cells were grouped based on their previously defined phenotypic features, and a gene expression pattern for each group of cell lines was established. The data indicated that the cell lines representing a defined stage generally presented a high similarity in overall expression profiles. Numerous genes could be identified as expressed with a restricted pattern using dCHIP-based, quantitative comparisons or presence/absence-based, probabilistic state analysis. These experiments provide a model for gene expression during B cell development, and the correctly identified expression patterns of a number of control genes suggest that a series of cell lines can be useful tools in the elucidation of the molecular genetics of a complex differentiation process.

Key Words: presence/absence • immunoglobulin • progenitor B cells

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