HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

Published online before print May 22, 2003

This Article Full Text (Reprint (PDF)) All Versions of this Article: jlb.0102016v1 74/2/260    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Willeke, T. Articles by Walzog, B. Search for Related Content PubMed PubMed Citation Articles by Willeke, T. Articles by Walzog, B.

© 2003 by The Society for Leukocyte Biology
Journal of Leukocyte Biology, doi:10.1189/jlb.0102016

Received for publication January 10, 2002.
Revised March 18, 2003.
Accepted for publication April 1, 2003.

Article

A role for Syk-kinase in the control of the binding cycle of the ₂ integrins (CD11/CD18) in human polymorphonuclear neutrophils

Thomas Willeke ^*, Jürgen Schymeinsky ^*, Peggy Prange , Stefan Zahler ^*, and Barbara Walzog ^*@

*Department of Physiology, Ludwig-Maximilians-Universität München, Germany; and Department of Physiology, Freie Universität Berlin, Germany

^@ To whom correspondence should be addressed. E-mail: walzog{at}lrz.uni-muenchen.de.

	Abstract

A fine control of ₂ integrin (CD11/CD18)-mediated firm adhesion of human neutrophils to the endothelial cell monolayer is required to allow ordered emigration. To elucidate the molecular mechanisms that control this process, intracellular protein tyrosine signaling subsequent to ₂ integrin-mediated ligand binding was studied by immunoprecipitation and Western blotting techniques. The 72-kDa Syk-kinase, which was tyrosine-phosphorylated upon adhesion, was found to coprecipitate with CD18, the -subunit of the ₂ integrins. Moreover, inhibition of Syk-kinase by piceatannol enhanced adhesion and spreading but diminished N-formyl-Met-Leu-Phe-induced chemotactic migration. The enhancement of adhesiveness was associated with integrin clustering, which results in increased integrin avidity. In contrast, piceatannol had no effect on the surface expression or on the affinity of ₂ integrins. Altogether, this suggests that Syk-kinase controls alternation of ₂ integrin-mediated ligand binding with integrin detachment, which is necessary for neutrophil extravasation by regulating integrin clustering.

Key Words: LFA-1 • ICAM-1 • SDS-PAGE

This article has been cited by other articles:

				S. Luangdilok, C. Box, L. Patterson, W. Court, K. Harrington, L. Pitkin, P. Rhys-Evans, P. O-charoenrat, and S. Eccles Syk Tyrosine Kinase Is Linked to Cell Motility and Progression in Squamous Cell Carcinomas of the Head and Neck Cancer Res., August 15, 2007; 67(16): 7907 - 7916. [Abstract] [Full Text] [PDF]

				J. Schymeinsky, A. Sindrilaru, D. Frommhold, M. Sperandio, R. Gerstl, C. Then, A. Mocsai, K. Scharffetter-Kochanek, and B. Walzog The Vav binding site of the non-receptor tyrosine kinase Syk at Tyr 348 is critical for beta2 integrin (CD11/CD18)-mediated neutrophil migration Blood, December 1, 2006; 108(12): 3919 - 3927. [Abstract] [Full Text] [PDF]

				Y. Shi, Y. Tohyama, T. Kadono, J. He, S. M. Shahjahan Miah, R. Hazama, C. Tanaka, K. Tohyama, and H. Yamamura Protein-tyrosine kinase Syk is required for pathogen engulfment in complement-mediated phagocytosis Blood, June 1, 2006; 107(11): 4554 - 4562. [Abstract] [Full Text] [PDF]

				A. Ortiz-Stern and C. Rosales Fc{gamma}RIIIB stimulation promotes {beta}1 integrin activation in human neutrophils J. Leukoc. Biol., May 1, 2005; 77(5): 787 - 799. [Abstract] [Full Text] [PDF]

				S. R. Yan, D. M. Byers, and R. Bortolussi Role of Protein Tyrosine Kinase p53/56lyn in Diminished Lipopolysaccharide Priming of Formylmethionylleucyl- phenylalanine-Induced Superoxide Production in Human Newborn Neutrophils Infect. Immun., November 1, 2004; 72(11): 6455 - 6462. [Abstract] [Full Text] [PDF]

				R. Kettritz, M. Choi, S. Rolle, M. Wellner, and F. C. Luft Integrins and Cytokines Activate Nuclear Transcription Factor-{kappa}B in Human Neutrophils J. Biol. Chem., January 23, 2004; 279(4): 2657 - 2665. [Abstract] [Full Text] [PDF]