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Originally published online as doi:10.1189/jlb.0309188 on September 10, 2009

Published online before print September 10, 2009
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(Journal of Leukocyte Biology. 2010;87:51-58.)
© 2010 Society for Leukocyte Biology

Enhanced production of IL-17A during zymosan-induced peritonitis in obese mice

Maria Pini and Giamila Fantuzzi1

Department of Kinesiology and Nutrition, University of Illinois at Chicago, Chicago, Illinois, USA

1. Correspondence: Department of Kinesiology and Nutrition, University of Illinois at Chicago, 1919 W. Taylor St., MC 517, Chicago, IL 60612, USA. E-mail: giamila{at}uic.edu

ABSTRACT

IL-17A is a proinflammatory cytokine critical for host defense and involved in the pathogenesis of autoimmune disorders. Obesity is associated with chronic low-grade inflammation but also with a heightened acute inflammatory response. We investigated the effect of obesity on IL-17A production using the model of ZY-induced peritonitis. Compared with lean controls, administration of ZY induced a significantly exacerbated inflammatory response in obese leptin-deficient ob/ob mice and in mice with diet-induced obesity (DIO). Levels of IL-17A in the peritoneal fluid in response to ZY were elevated significantly in ob/ob and DIO mice compared with lean animals. Reconstitution of ob/ob mice with exogenous leptin did not alter production of IL-17A significantly in response to ZY. Peritoneal cells and adipose tissue obtained from ZY-injected obese mice expressed significantly higher levels of IL-17A mRNA compared with lean mice. Approximately 2% of peritoneal Ly6G+ neutrophils from ZY-injected obese mice expressed IL-17A protein, compared with 0.2% of cells obtained from lean mice. Neutralization of IL-17 in ob/ob mice inhibited neutrophil recruitment and production of neutrophil-attracting CXC chemokines and IL-6, without affecting macrophage infiltration or levels of IL-10 and the chemokine CCL2. In contrast, neutralization of IL-6 did not affect production of IL-17A or chemokines while reducing production of the acute-phase protein serum amyloid A significantly. These data demonstrate that neutrophil-derived IL-17A is increased in obese mice during acute inflammation and contributes to exacerbation of inflammatory responses.

Key Words: neutrophils • cytokines • inflammation • rodent


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