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Originally published online as doi:10.1189/JLB.1008639 on June 18, 2009

Published online before print June 18, 2009
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(Journal of Leukocyte Biology. 2009;86:691-699.)
© 2009 Society for Leukocyte Biology

Synergistic production of interleukin-23 by dendritic cells derived from cord blood in response to costimulation with LPS and IL-12

Mi Seon Jang*, Young Min Son*, Gi Rak Kim*, Yeo Jin Lee*, Woon Kyu Lee§, Seok Ho Cha{dagger}, Seung Hyun Han{ddagger} and Cheol-Heui Yun*,1

* Protein Engineering and Comparative Immunology, Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, and
{ddagger} Department of Oral Microbiology and Immunology, BK21 Program, and Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Republic of Korea; and
{dagger} Department of Pharmacology and Toxicology and
§ Center for Advanced Medical Education by BK21 Project, College of Medicine, Inha University, Incheon, Republic of Korea

1. Correspondence: Protein Engineering and Comparative Immunology, Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of Korea. E-mail: cyun{at}snu.ac.kr

ABSTRACT

This study was performed to provide insight for the optimization and regulation of immune homeostasis, which should be taken into account in the development of cell therapy using DCs and/or cytokine. Human CBDCs costimulated with LPS and IL-12 were examined for cytokine expression compared with ABDCs. Our results showed that costimulation with IL-12 and LPS in CBDCs resulted in increased expression of IL-23. Concomitantly, the phosphorylation of ERKs and p38 MAPK was increased, suggesting that these kinases are important signaling components for IL-23 induction in CBDC costimulated with LPS and IL-12. Furthermore, production of IL-23 in CBDC costimulated with LPS and IL-12 caused CD4+CD45RO+ memory cells to increase IFN-{gamma} production. Taken together, CBDCs, costimulated with LPS and IL-12, show a synergistic increase in IL-23 production via enhanced phosphorylation of ERK1/2 and p38 MAPK and consequently, an induction of IFN-{gamma} production in the memory cells.

Key Words: human • antigen-presenting cells • cytokines