Miltenyi Biotec GmbH
Originally published online as doi:10.1189/jlb.1008591 on April 28, 2009

Published online before print April 28, 2009
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(Journal of Leukocyte Biology. 2009;86:389-399.)
© 2009 Society for Leukocyte Biology

TLR2 ligands augment cPLA2{alpha} activity and lead to enhanced leukotriene release in human monocytes

Sabine C. Lindner*, Ulrike Köhl{dagger}, Thorsten J. Maier*, Dieter Steinhilber* and Bernd L. Sorg*,1

* Institute of Pharmaceutical Chemistry/Center for Drug Research, Development, and Safety (ZAFES), and
{dagger} Pediatric Hematology and Oncology, Goethe University, Frankfurt/Main, Germany

1. Correspondence: Institute of Pharmaceutical Chemistry/Center for Drug Research, Development, and Safety (ZAFES), Goethe University, Max-von-Laue-Strasse 9, 60438 Frankfurt/Main, Germany. E-mail: b.sorg{at}pharmchem.uni-frankfurt.de

ABSTRACT

Toll-like receptors (TLRs) play an important role in innate immunity. They detect pathogen-associated receptor patterns (PAMPs) and initiate subsequent immune responses. Present studies investigate the influence of TLR2 ligands on leukotrienes (LT) formation in human monocytes. LTs are proinflammatory mediators derived from arachidonic acid (AA), which is released from membranes by phospholipase A2 (PLA2) enzymes. Pretreatment of MM6 cells with the TLR2 ligands LTA, FSL-1, or Pam3CSK4 resulted in an up to two- to threefold enhancement of ionophore-induced LT formation in a dose- and time-dependent manner and to an augmentation of ionophore-induced AA release with similar kinetics. Also in human peripheral blood mononuclear cells (hPBMC), TLR2 activators increased LT formation. Studies with PLA2 inhibitors indicated that the increase of AA release is a result of enhanced activity of group IV cPLA2 in MM6 cells. TLR2 ligands elicited the time-dependent activation of p38 MAPK and ERK1/2 pathways, which led to phosphorylation of cPLA2{alpha} at Ser505. Simultaneous inhibition of p38 MAPK and ERK1/2 pathways prevented the increase of cPLA2{alpha} phosphorylation and the augmentation of AA release. TLR2 ligand-induced increase of AA release was blocked by a neutralizing anti-hTLR2 antibody, indicating that TLR2 mediates augmented cPLA2 activation and subsequent LT biosynthesis.

Key Words: innate immunity • arachidonic acid • 5-lipoxygenase • Mono Mac 6