Accuri C6 Flow Cytometer System
Originally published online as doi:10.1189/jlb.0208126 on May 4, 2009

Published online before print May 4, 2009
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(Journal of Leukocyte Biology. 2009;86:361-370.)
© 2009 Society for Leukocyte Biology

Chorionic gonadotropin alleviates thioglycollate-induced peritonitis by affecting macrophage function

Hui Wan*,1, Jojanneke M. C. Coppens*, Cornelia G. van Helden-Meeuwsen*, Pieter J. M. Leenen*, Nico van Rooijen{dagger}, Nisar A. Khan*, Rebecca C. M. Kiekens*, Robbert Benner* and Marjan A. Versnel*

* Department of Immunology, Erasmus MC, Rotterdam, The Netherlands; and
{dagger} Department of Molecular Cell Biology, VUMC, Amsterdam, The Netherlands

1. Correspondence: Department of Immunology, Erasmus MC, Dr. Molewaterplein 50, Rotterdam, Zuid Holland 3015 GE, The Netherlands. E-mail: h.wan{at}erasmusmc.nl

ABSTRACT

Human chorionic gonadotrophin (hCG) is a hormone produced during pregnancy and present at the implantation site and in the maternal blood. Pregnancy has been proposed to represent a controlled state of inflammation at an early stage at the implantation site and later, systemically extended to the maternal circulation. Earlier, we reported that hCG can inhibit the development of diabetes in NOD mice and LPS-induced septic shock in a murine model. We hypothesize that hCG can contribute to the reduction of inflammation by modifying M{varphi} function. Here, the TG-induced peritonitis model for inflammation was used to investigate the effect of hCG on cytokine production and cell recruitment in vivo. hCG pretreatment in TG-induced peritonitis increased the number of peritoneal cells, especially PMN and monocytes, compared with mice injected with TG only. This increased cell number was partially explained by increased cell survival induced by hCG. Despite the cellular infiltrate, hCG pretreatment decreased i.p. TNF-{alpha}, IL-6, PTX3, CCL3, and CCL5 levels. By depleting peritoneal resident M{varphi} using clodronate liposomes prior to the application of hCG and the TG trigger, we established that M{varphi} are the main responsive cells to hCG, as the suppressed TNF-{alpha} and IL-6 production and increased PMN influx are abolished in their absence. Together, these data suggest that hCG contributes to the controlled inflammatory state of pregnancy by regulating M{varphi} proinflammatory function.

Key Words: hCG • resident M{varphi} • cell recruitment • clodronate liposomes