Pepro Tech
Originally published online as doi:10.1189/jlb.0708429 on February 6, 2009

Published online before print February 6, 2009
This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow All Versions of this Article:
jlb.0708429v1
85/6/911    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Guo, H.
Right arrow Articles by Zhang, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Guo, H.
Right arrow Articles by Zhang, N.
(Journal of Leukocyte Biology. 2009;85:911-918.)
© 2009 by Society for Leukocyte Biology

Pivotal Advance: PKC{zeta} is required for migration of macrophages

Hua Guo*,{dagger},1, Yongjie Ma*,1, Baogang Zhang*, Baocun Sun*, Ruifang Niu*, Guoguang Ying* and Ning Zhang*,2

* Tianjin Medical University Cancer Institute and Hospital, Research Center of Basic Medical Sciences, Tianjin, China; and
{dagger} Tianjin Medical College, Tianjin, China

2 Correspondence: Tianjin Medical University, Research Center of Basic Medical Sciences and Cancer Institute and Hospital, Tianjin, 300060, China. E-mail: zhangning{at}tijmu.edu.cn

ABSTRACT

The crosstalk, mediated by chemoattractants, between cancer cells and tumor-associated macrophages, plays an important role in tumor invasion and metastasis. Our previous study reported that atypical protein kinase C {zeta} (PKC{zeta}) regulates epidermal growth factor-induced chemotaxis of human breast cancer cells. In this study, we investigated the role of PKC{zeta} in CSF-1-induced chemotaxis of macrophages. Knockdown of PKC{zeta} by small interference RNA impaired CSF-1-induced chemotaxis of human acute monocytic leukemia cell line THP-1, which was probably a result of a decrease in CSF-1-induced phosphorylation of LIN-11, Is11, and MEC-3 protein domain kinase (LIMK)/cofilin and actin polymerization. Furthermore, silencing PKC{zeta} expression also impaired migration of mouse peritoneal macrophages. Scratch analysis indicated that PKC{zeta} was required for macrophage migration. Therefore, PKC{zeta} is required for CSF-1-induced chemotaxis of macrophages. Blocking activation of PKC{zeta} will be a novel strategy to inhibit cancer metastasis by blocking migration of cancer cells and macrophages.

Key Words: CSF-1 • chemokine • metastasis • tumor-associated macrophages




This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
H. Yao, J.-w. Hwang, J. Moscat, M. T. Diaz-Meco, M. Leitges, N. Kishore, X. Li, and I. Rahman
Protein Kinase C{zeta} Mediates Cigarette Smoke/Aldehyde- and Lipopolysaccharide-induced Lung Inflammation and Histone Modifications
J. Biol. Chem., February 19, 2010; 285(8): 5405 - 5416.
[Abstract] [Full Text] [PDF]