Accuri C6 Flow Cytometer System
Originally published online as doi:10.1189/jlb.0708394 on October 29, 2008

Published online before print October 29, 2008
This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow All Versions of this Article:
jlb.0708394v1
85/2/289    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Stevenson, N. J.
Right arrow Articles by Johnston, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Stevenson, N. J.
Right arrow Articles by Johnston, J. A.
(Journal of Leukocyte Biology. 2009;85:289-297.)
© 2009 Society for Leukocyte Biology

CCL11 blocks IL-4 and GM-CSF signaling in hematopoietic cells and hinders dendritic cell differentiation via suppressor of cytokine signaling expression

Nigel J. Stevenson*,1, Mark R. Addley{dagger},1, Elizabeth J. Ryan{ddagger}, Caroline R. Boyd*, Helen P. Carroll*, Verica Paunovic*, Christina A. Bursill{dagger},§, Helen C. Miller{dagger}, Keith M. Channon§, Angela E. McClurg*, Marilyn A. Armstrong*, Wilson A. Coulter||, David R. Greaves{dagger},2 and James A. Johnston*,2,3

* Infection and Immunity, Cancer Research and Cell Biology, School of Biomedical Science, and
|| School of Dentistry, Queen’s University of Belfast, Belfast, Northern Ireland;
{dagger} Sir William Dunn School of Pathology and
§ Department of Cardiovascular Medicine, John Radcliffe Hospital, University of Oxford, Oxford, United Kingdom; and
{ddagger} Department of Immunology and Biochemistry, Trinity College Dublin, Dublin, Ireland

3 Correspondence: Infection and Immunity, CCRCB, School of Biomedical Science, Queen’s University of Belfast, Belfast BT9 7BL, Northern Ireland. E-mail: jim.johnston{at}qub.ac.uk

The chemokine eotaxin/CCL11 is an important mediator of leukocyte migration, but its effect on inflammatory cytokine signaling has not been explored. In this study, we find that CCL11 induces suppressor of cytokine signaling (SOCS)1 and SOCS3 expression in murine macrophages, human monocytes, and dendritic cells (DCs). We also discover that CCL11 inhibits GM-CSF-mediated STAT5 activation and IL-4-induced STAT6 activation in a range of hematopoietic cells. This blockade of cytokine signaling by CCL11 results in reduced differentiation and endocytic ability of DCs, implicating CCL11-induced SOCS as mediators of chemotactic inflammatory control. These findings demonstrate cross-talk between chemokine and cytokine responses, suggesting that myeloid cells tracking to the inflammatory site do not differentiate in the presence of this chemokine, revealing another role for SOCS in inflammatory regulation.

Key Words: dendritic cells • monocytes/macrophages • chemokines • signal transduction