Accuri C6 Flow Cytometer System
Originally published online as doi:10.1189/jlb.0508310 on November 13, 2008

Published online before print November 13, 2008
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(Journal of Leukocyte Biology. 2009;85:268-277.)
© 2009 The Author(s)

Differential impact of L-arginine deprivation on the activation and effector functions of T cells and macrophages

B.-S. Choi*, I. Clara Martinez-Falero*, C. Corset*, M. Munder{dagger}, M. Modolell{ddagger}, I. Müller* and P. Kropf*,1

* Department of Immunology, Faculty of Medicine, Imperial College London, United Kingdom;
{dagger} Department of Hematology, Oncology, and Rheumatology, University Hospital Heidelberg, Heidelberg, Germany; and
{ddagger} Department of Cellular Immunology, Max-Planck-Institute for Immunobiology, Freiburg, Germany

1 Correspondence: Imperial College London, Department of Immunology, Norfolk Place, London W2 1PG, UK. E-mail: p.kropf{at}imperial.ac.uk

The metabolism of the amino acid L-arginine is emerging as a crucial mechanism for the regulation of immune responses. Here, we characterized the impact of L-arginine deprivation on T cell and macrophage (M{Phi}) effector functions: We show that whereas L-arginine is required unconditionally for T cell activation, M{Phi} can up-regulate activation markers and produce cytokines and chemokines in the absence of L-arginine. Furthermore, we show that L-arginine deprivation does not affect the capacity of activated M{Phi} to up-regulate L-arginine-metabolizing enzymes such as inducible NO synthase and arginase 1. Thus, our results show that to exert their effector functions, T cells and M{Phi} have different requirements for L-arginine.

Key Words: arginase • immune regulation • t cell hyporesponsiveness • iNOS • cytokine • chemokine