Pepro Tech
Originally published online as doi:10.1189/jlb.0508327 on September 10, 2008

Published online before print September 10, 2008
This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow All Versions of this Article:
jlb.0508327v1
84/6/1492    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Qian, K.
Right arrow Articles by Wu, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Qian, K.
Right arrow Articles by Wu, J.
(Journal of Leukocyte Biology. 2008;84:1492-1500.)
© 2008 by Society for Leukocyte Biology

Functional expression of IgA receptor Fc{alpha}RI on human platelets

Kun Qian1, Fenglong Xie, Andrew W. Gibson, Jeffrey C. Edberg, Robert P. Kimberly and Jianming Wu2

Division of Clinical Immunology and Rheumatology, Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama, USA

2 Correspondence: Division of Clinical Immunology and Rheumatology, Department of Medicine, University of Alabama at Birmingham, 202 Shelby Interdisciplinary Biomedical Science Building, 1825 University Boulevard, Birmingham, AL 35294-2182, USA. E-mail: jmwu{at}uab.edu

Fc{alpha}RI (CD89) is a human IgA FcR expressed on cells of myeloid lineage such as neutrophils, monocytes, tissue macrophages, eosinophils, and subpopulations of dendritic cells. Fc{alpha}RI mediates cell activation through Src family kinases and downstream tyrosine-based phosphorylation pathways. However, the role of IgA and the expression and role of its cognate receptor Fc{alpha}RI (CD89) in platelet activation are undefined. In the current study, we demonstrate that human platelets express Fc{alpha}RI mRNAs and proteins. Furthermore, we show that the platelet Fc{alpha}RI is associated with the FcR {gamma}-chain, and cross-linking of Fc{alpha}RI leads to Syk phosphorylation. Clustering of Fc{alpha}RI induces pre-mRNA splicing and protein production of tissue factor and IL-1β, suggesting novel roles for human platelet Fc{alpha}RI and serum IgA in thrombosis and inflammation.

Key Words: CD89 • pre-mRNA splicing • IL-1_oblique>β • tissue factor