Journal of Leukocyte Biology
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Originally published online as doi:10.1189/jlb.1107802 on May 21, 2008

Published online before print May 21, 2008
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(Journal of Leukocyte Biology. 2008;84:448-459.)
© 2008 by Society for Leukocyte Biology

CD13 is a novel mediator of monocytic/endothelial cell adhesion

Paola Mina-Osorio*, Beata Winnicka*, Catherine O'Conor*, Christina L. Grant*, Lotte K. Vogel{dagger}, Daniel Rodriguez-Pinto*, Kathryn V. Holmes{ddagger}, Enrique Ortega§ and Linda H. Shapiro*,1

* Center for Vascular Biology, Department of Cell Biology, University of Connecticut Health Center, Farmington, Connecticut, USA;
{dagger} Department of Medical Biochemistry and Genetics, The Panum Institute, University of Copenhagen. Denmark;
{ddagger} Molecular Biology Program, University of Colorado at Denver and Health Sciences Center, Aurora, Colorado, USA; and
§ Department of Immunology, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México

1 Correspondence: University of Connecticut Health Center, 263 Farmington Ave., Farmington, CT 06030-3501, USA. E-mail: lshapiro{at}neuron.uchc.edu

During inflammation, cell surface adhesion molecules guide the adhesion and migration of circulating leukocytes across the endothelial cells lining the blood vessels to access the site of injury. The transmembrane molecule CD13 is expressed on monocytes and endothelial cells and has been shown to mediate homotypic cell adhesion, which may imply a role for CD13 in inflammatory monocyte trafficking. Here, we show that ligation and clustering of CD13 by mAb or viral ligands potently induce myeloid cell/endothelial adhesion in a signal transduction-dependent manner involving monocytic cytoskeletal rearrangement and filopodia formation. Treatment with soluble recombinant (r)CD13 blocks this CD13-dependent adhesion, and CD13 molecules from monocytic and endothelial cells are present in the same immunocomplex, suggesting a direct participation of CD13 in the adhesive interaction. This concept is strengthened by the fact that activated monocytic cells adhere to immobilized recombinant CD13. Furthermore, treatment with anti-CD13 antibodies in a murine model of peritonitis results in a decrease in leukocyte infiltration into the peritoneum, suggesting a potential role for CD13 in leukocyte trafficking in vivo. Therefore, this work supports a new direction for CD13 biology, where these cell surface molecules act as true molecular interfaces that induce and participate in critical inflammatory cell interactions.

Key Words: aminopeptidase • monocyte activation • inflammation • leukocyte trafficking






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